Test
Thermo Fisher Scientific offers bright conjugates of wheat germ agglutinin (WGA) and Alexa Fluor, Alexa Fluor Plus, and other dyes. Fluorescent WGA conjugates bind to carbohydrates and are used for various cell biology applications such as plasma membrane labeling and cell painting assays.
Ligand Type | N-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues |
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Protein Family | Lectins |
Protein Form | Heterodimer |
Expression System | Wheat germ |
For Use With (Application) | Flow Cytometry,Immunocytochemistry,Immunofluorescence,Immunohistochemistry,Cell Painting |
Protein Tag | None |
Species | Wheat |
Protein | Fluorescent lectins |
Protein Subtype | Agglutinin |
Thermo Fisher Scientific offers a broad selection of Invitrogen concanavalin A (Con A) conjugates, including Alexa Fluor™ and Alexa Fluor™ Plus conjugates.
Shipping Condition | Room Temperature |
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Ligand Type | Lectin |
Protein Family | Lectins |
Purity or Quality Grade | Imaging Quality |
Form | Lyophilized |
Research Category | Imaging |
Cross Reactivity | α-man, α-glc |
For Use With (Application) | Immunocytochemistry,Immunohistochemistry,Immunofluorescence |
Protein Tag | None |
Species | C. ensiformis |
Source | Canavlia ensiformis |
Recombinant | Native |
Lycopersicon esculentum lectin (LEL or tomato lectin, TL) is a single-subunit glycoprotein containing arabinose and galactose and may form multimeric aggregates in solution. Although it shares specificities with potato lectin, Datura lectin, and wheat germ agglutinin, LEL is dissimilar in many respects. It binds well to glycophorin and Tamm-Horsfall glycoprotein, has been used to label vascular endothelium in rodents, and is an effective marker of blood vessels and microglial cells in rodents. Conjugation with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections. This fluorescein-labeled LEL features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 495 nm, emission 515 nm). Supplied as a solution essentially free of unconjugated fluorophores, it is preserved with sodium azide. The recommended inhibiting/eluting sugar is chitin hydrolysate.
Vicia villosa lectin (VVL, VVA) preferentially binds α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen). This lectin may require specific amino acid sequences at the receptor site of glycosylation, and the disaccharide galactosyl (α-1,3) N-acetylgalactosamine is also a potent inhibitor. This conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
Sambucus nigra lectin, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in α-2,6 and to a lesser degree, α-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin appears to bind sialic acid linked to N-acetylgalactosamine or galactose. SNA has been reported to inhibit cell-free protein synthesis. 2mg; Solution: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Our lectin screening kits include seven lectins or lectin conjugates. These lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually. Each Kit I (BK-1000, FLK-2100, RLK-2200) consists of 1 mg of the following lectins or lectin conjugates: Con A, DBA, PNA, RCA120 ,SBA, UEA I, WGA. These bioinylated conjugates are prepared from our affinity-purified lectins and are optimally labeled with biotin. Essentially free of inactive lectin conjugates and containing no free biotin, these biotinylated lectins are ideal intermediates for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First a biotin-labeled lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections. Fluorescent dye-labeled tomato lectin is a valuable tool for studying rodent tumor angiogenesis, tracing neovascular development in xenograft models and brain research. DyLight 488-conjugated tomato lectin fluoresces green (emission maximum 518 nm) and has an excitation maximum at 493 nm. Inhibiting/Eluting Sugar: Chitin Hydrolysate
Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections. Fluorescent dye-labeled tomato lectin is a valuable tool for studying rodent tumor angiogenesis, tracing neovascular development in xenograft models and brain research. DyLight 594-labeled tomato lectin emits in red and is an excellent second label in systems with green/yellow fluorescence such as GFP expressed in transgenic animals, or with fluorescein conjugates in standard double-label studies. Excitation maximum: 592 nmEmission maximum: 617 nmColor: Red. Inhibiting/Eluting Sugar: Chitin Hydrolysate
Concanavalin A (ConA) recognizes α-linked mannose residues, which are present as part of a "core oligosaccharide" in many serum and membrane-bound glycoproteins. This biotinylated ConA conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
Sambucus nigra lectin (SNL) is isolated from elderberry bark and binds preferentially to sialic acid attached to terminal galactose in a-2,6 and to a lesser degree, a-2,3 linkage. Also called SNA (Sambucus nigra agglutinin) and EBL (elderberry lectin), SNL can inhibit cell-free protein synthesis. This agarose-bound SNL is prepared using affinity-purified lectin and heat stable, cross-linked 4% agarose beads. The attachment of the lectin through a hydrophilic spacer arm to the beads is carefully controlled to preserve the activity of and minimize conformational changes to the bound lectins. The recommended inhibiting/eluting sugar is 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid.
Sambucus nigra lectin (SNL) is isolated from elderberry bark and binds preferentially to sialic acid attached to terminal galactose in a-2,6 and to a lesser degree, a-2,3 linkage. Also called SNA (Sambucus nigra agglutinin) and EBL (elderberry lectin), SNL can inhibit cell-free protein synthesis. Binding is also inhibited to some extent by lactose or galactose. This lectin does not appear to bind sialic acid linked to N-acetylgalactosamine. SNA has been reported to inhibit cell-free protein synthesis. This fluorescein-labeled SNL features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 495 nm, emission 515 nm). Supplied as a solution essentially free of unconjugated fluorophores, it is preserved with sodium azide. The recommended inhibiting/eluting sugar is 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid.
Artocarpus integrifolia (Jacalin) Lectin isolated from Artocarpus integrifolia (jackfruit) seeds. Specifications: Primary sugar specificity: Galactose Preferential binding activity: galactosyl (beta-1,4) N-acetylglucosamine.
ERYTHRINA (CORAL TREE) - LECTIN: Erythrina cristagalli (coral tree) Lectin isolated from Erythrina cristagalli (coral tree) seeds. Description: Agglutination activity is expressed in mg/ml and is determined from serial dilutions in p...
Technical Specifications:Ligand Source:Glycine maxMolecular Weight:120 kDaCarbohydrate Specificity:GalNAc, GalNAcα1,3GalInhibiting/Eluting Sugar:N-AcetylgalactosamineCarbohydrate for Elution:0.2MN-AcetylgalactosamineBlood Group Specificity:A>O>BConjugate: RhodamineForm:LiquidIsoelectric (pI):5.8-6Storage: -20°CApplication note: Calcium and Manganese are required for binding.
Technical Specifications:Ligand Source:Lens culinarisMolecular Weight:50 kDaCarbohydrate Specificity:αMan (High Mannose), Fucα6GlcNAcInhibiting/Eluting Sugar:a-Methylglucoside +a-MethylmannosideCarbohydrate for Elution:0.2Ma-Methylglucoside +a-MethylmannosideBlood Group Specificity:N/AConjugate: Texas RedForm:LiquidIsoelectric (pI):7.6-8.4Storage: -20°CApplication note: Calcium and Manganese are required for binding.