Test
Thermo Fisher Scientific offers bright conjugates of wheat germ agglutinin (WGA) and Alexa Fluor, Alexa Fluor Plus, and other dyes. Fluorescent WGA conjugates bind to carbohydrates and are used for various cell biology applications such as plasma membrane labeling and cell painting assays.
Ligand Type | N-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues |
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Protein Family | Lectins |
Protein Form | Heterodimer |
Expression System | Wheat germ |
For Use With (Application) | Flow Cytometry,Immunocytochemistry,Immunofluorescence,Immunohistochemistry,Cell Painting |
Protein Tag | None |
Species | Wheat |
Protein | Fluorescent lectins |
Protein Subtype | Agglutinin |
Thermo Fisher Scientific offers a broad selection of Invitrogen concanavalin A (Con A) conjugates, including Alexa Fluor™ and Alexa Fluor™ Plus conjugates.
Shipping Condition | Room Temperature |
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Ligand Type | Lectin |
Protein Family | Lectins |
Purity or Quality Grade | Imaging Quality |
Form | Lyophilized |
Research Category | Imaging |
Cross Reactivity | α-man, α-glc |
For Use With (Application) | Immunocytochemistry,Immunohistochemistry,Immunofluorescence |
Protein Tag | None |
Species | C. ensiformis |
Source | Canavlia ensiformis |
Recombinant | Native |
Affinity purified Maackia amurensis lectin (MAA) is a tetramer formed by MAA1 or MAL (a 37kD subunit called Maackia amurensis leukoagglutinin) and MAA2 or MAH (a 32 kD subunit called Maackia amurensis hemagglutinin).Technical Specifications:Ligand Source:Maackia amurensisMolecular Weight: 130 kDaCarbohydrate Specificity:Siaα1,3Galβ4GalNAcInhibiting/Eluting Sugar:LactoseCarbohydrate for Elution: 0.2MLactoseBlood Group Specificity: N/AConjugate: FITCForm: LiquidIsoelectric (pI): 4.7Storage: -20°CApplication note: N/A
Technical Specifications:Ligand Source:Maackia amurensisMolecular Weight:130 kDaCarbohydrate Specificity:Siaα1,3Galβ4GalNAcInhibiting/Eluting Sugar:LactoseCarbohydrate for Elution:0.2MLactoseBlood Group Specificity:N/AConjugate: RhodamineForm:LiquidIsoelectric (pI):4.7Storage: -20°CApplication note: N/A
Pure Maackia amurensis lectin (MAA), Texas Red ConjugatedTechnical Specifications:Ligand Source:Maackia amurensisMolecular Weight:130 kDaCarbohydrate Specificity:Siaα1,3Galβ4GalNAcInhibiting/Eluting Sugar:LactoseCarbohydrate for Elution:0.2MLactoseBlood Group Specificity:N/AConjugate: Texas RedForm:LiquidIsoelectric (pI):4.7Storage: -20°CApplication note: N/A
Sambucus Nigra Lectin - Rhodamine:
Sambucus Nigra Lectin - Texas Red:
Lycopersicon esculentum lectin (LEL or tomato lectin, TL) is a single-subunit glycoprotein containing arabinose and galactose and may form multimeric aggregates in solution. Although it shares specificities with potato lectin, Datura lectin, and wheat germ agglutinin, LEL is dissimilar in many respects. It binds well to glycophorin and Tamm-Horsfall glycoprotein, has been used to label vascular endothelium in rodents, and is an effective marker of blood vessels and microglial cells in rodents. Conjugation with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections. This fluorescein-labeled LEL features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 495 nm, emission 515 nm). Supplied as a solution essentially free of unconjugated fluorophores, it is preserved with sodium azide. The recommended inhibiting/eluting sugar is chitin hydrolysate.
Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections. Fluorescent dye-labeled tomato lectin is a valuable tool for studying rodent tumor angiogenesis, tracing neovascular development in xenograft models and brain research. DyLight 488-conjugated tomato lectin fluoresces green (emission maximum 518 nm) and has an excitation maximum at 493 nm. Inhibiting/Eluting Sugar: Chitin Hydrolysate
Vicia villosa lectin (VVL, VVA) preferentially binds α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen). This lectin may require specific amino acid sequences at the receptor site of glycosylation, and the disaccharide galactosyl (α-1,3) N-acetylgalactosamine is also a potent inhibitor. This conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
Sambucus nigra lectin, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in α-2,6 and to a lesser degree, α-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin appears to bind sialic acid linked to N-acetylgalactosamine or galactose. SNA has been reported to inhibit cell-free protein synthesis. 2mg; Solution: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Our lectin screening kits include seven lectins or lectin conjugates. These lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually. Each Kit I (BK-1000, FLK-2100, RLK-2200) consists of 1 mg of the following lectins or lectin conjugates: Con A, DBA, PNA, RCA120 ,SBA, UEA I, WGA. These bioinylated conjugates are prepared from our affinity-purified lectins and are optimally labeled with biotin. Essentially free of inactive lectin conjugates and containing no free biotin, these biotinylated lectins are ideal intermediates for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First a biotin-labeled lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections. Fluorescent dye-labeled tomato lectin is a valuable tool for studying rodent tumor angiogenesis, tracing neovascular development in xenograft models and brain research. DyLight 594-labeled tomato lectin emits in red and is an excellent second label in systems with green/yellow fluorescence such as GFP expressed in transgenic animals, or with fluorescein conjugates in standard double-label studies. Excitation maximum: 592 nmEmission maximum: 617 nmColor: Red. Inhibiting/Eluting Sugar: Chitin Hydrolysate
Concanavalin A (ConA) recognizes α-linked mannose residues, which are present as part of a "core oligosaccharide" in many serum and membrane-bound glycoproteins. This biotinylated ConA conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
Sambucus nigra lectin (SNL) is isolated from elderberry bark and binds preferentially to sialic acid attached to terminal galactose in a-2,6 and to a lesser degree, a-2,3 linkage. Also called SNA (Sambucus nigra agglutinin) and EBL (elderberry lectin), SNL can inhibit cell-free protein synthesis. Binding is also inhibited to some extent by lactose or galactose. This lectin does not appear to bind sialic acid linked to N-acetylgalactosamine. SNA has been reported to inhibit cell-free protein synthesis. This fluorescein-labeled SNL features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 495 nm, emission 515 nm). Supplied as a solution essentially free of unconjugated fluorophores, it is preserved with sodium azide. The recommended inhibiting/eluting sugar is 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid.