Restriction Enzymes

Restriction Enzymes

Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.
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1 – 24 of 24 results
1

OPTIZYME™ RsaI, Fisher BioReagents™

2

OPTIZYME™ ClaI (Bsu15I), Fisher BioReagents™

Components >50% Water, 25 to 50% Glycerin
Cut Site AT.CGAT
Content And Storage Keep container tightly closed
Form Liquid
Product Type ClaI (Bsu15I)
Storage Buffer 10mM Tris HCl (pH 8 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
pH 7.4
Incubator Temperature 37°C
Concentration 10 U/μL
3

OPTIZYME™ BpiI (BbsI), Fisher BioReagents™

4

OPTIZYME™ HpaII, Fisher BioReagents™

5

OPTIZYME™ XbaI, Fisher BioReagents™

Components 10X OPTIZYME™ Buffer 4
Cut Site T.CTAGA
Form Liquid
Product Type XbaI
Storage Buffer 10mM Tris HCl (pH 7.4), 100mM KCl, 7mM 2-mercaptoethano, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
pH 7.4
Incubator Temperature 37°C
For Use With (Application) Indicates ligation efficiency of DNA fragments generated by digestion with the Restriction Enzyme
6

OPTIZYME™ DdeI (HpyF31), Fisher BioReagents™

Components 25 to 50% Water, >50% Glycerin
Cut Site C.TNAG
Content And Storage Keep container tightly closed
Form Liquid
Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
pH 7.4
Incubator Temperature 37°C
Concentration 10 U/μL
For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI
7

OPTIZYME™ HinfI, Fisher BioReagents™

8

OPTIZYME™ EcoRI, Fisher BioReagents™

Components 25 to 50% Water, >50% Glycerin, 2.5 to10% Sodium Chloride
Cut Site G.AATTC
Content And Storage Keep container tightly closed
Form Liquid
Product Type EcoRI
Storage Buffer 10mM Potassium Phosphate (pH 7.4 at 25°C), 300mM NaCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 0.15% Triton™ X-100, 50% Glycerol
pH 7.4
Incubator Temperature 37°C
For Use With (Application) >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with EcoRI
9

OPTIZYME™ BglII, Fisher BioReagents™

Components 25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
Cut Site A.GATCT
Content And Storage Keep container tightly closed
Form Liquid
Product Type BglII
Storage Buffer 10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
pH 7.4
Incubator Temperature 37°C
Concentration 10 U/μL
For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII
10

OPTIZYME™ SacI, Fisher BioReagents™

11

OPTIZYME™ HindIII, Fisher BioReagents™

Components 25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride
Cut Site A.AGCTT
Content And Storage Keep container tightly closed
Form Liquid
Product Type HindIII
Storage Buffer 10mM Tris HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Incubator Temperature 37°C
Concentration 10 U/μL
For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII
12

OPTIZYME™ BglI, Fisher BioReagents™

13

OPTIZYME™ NotI, Fisher BioReagents™

Components 25 to 50% Water, >50% Glycerin
Cut Site GC.GGCCGC
Content And Storage Keep container tightly closed
Form Liquid
Product Type NotI
Storage Buffer 20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
pH 7.4
Concentration 10 U/μL
For Use With (Application) >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I
14

OPTIZYME™ AloI, Fisher BioReagents™

15

OPTIZYME™ BamHI, Fisher BioReagents™

Components 25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
Cut Site G.GATCC
Content And Storage Keep container tightly closed
Form Liquid
Product Type BamHI
Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
pH 7.4
Incubator Temperature 37°C
Concentration 10 U/μL
For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BamHI
16

OPTIZYME™ MspI (HpaII), Fisher BioReagents™

17

OPTIZYME™ SmaI, Fisher BioReagents™

Components 10X OPTIZYME™ Buffer 4
Cut Site CCC.GGG
Form Liquid
Product Type SmaI
Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
pH 7.4
Incubator Temperature 30°C
Concentration 10 U/μL
For Use With (Application) >90% of pUC19 DNA fragments can be ligated and re-cut after 50-fold over-digestion with SmaI
18

OPTIZYME™ NsiI (Mph1103I) Fisher BioReagents™

19

OPTIZYME™ XmaJI (AvrII), Fisher BioReagents™

20

OPTIZYME™ SpeI (BcuI), Fisher BioReagents™

21

OPTIZYME™ AarI, Fisher BioReagents™

22

OPTIZYME™ Csp6I (CviQI), Fisher BioReagents™

23

Fisher BioReagents™ Restriction Enzymes - Hae III (Source: Haemophilus aegyptius)

24

OPTIZYME™ HaeIII (BsuRI), Fisher BioReagents™