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OPTIZYME™ RsaI, Fisher BioReagents™
Description
5'...G T^A C...3'
3'...C A^T G...5'
Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 50 - 100%
- Buffer 2: 20 - 50%
- Buffer 3: 0 - 20%
- Buffer 4: 100%
- Buffer 5: 0 - 20%
- 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
- More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with RsaI
- 5'...GTAm5C G...3'
- 5'...m5C GTAm5C G...3'
Specifications
10 U/μL | |
37°C | |
>90% of DNA fragments can be ligated and re-cut after 10-fold over-digestion with Rsa I | |
Keep container tightly closed | |
GT.AC | |
Liquid |
>50% Glycerin, 25 to 50% Water | |
7.4 | |
10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol | |
1000 U | |
RsaI |
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