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OPTIZYME™ EcoRI, Fisher BioReagents™
Specifications
Components | 25 to 50% Water, >50% Glycerin, 2.5 to10% Sodium Chloride |
---|---|
Incubator Temperature | 37°C |
pH | 7.4 |
For Use With (Application) | >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with EcoRI |
Storage Buffer | 10mM Potassium Phosphate (pH 7.4 at 25°C), 300mM NaCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 0.15% Triton™ X-100, 50% Glycerol |
Catalog Number | Concentration | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Catalog Number | Concentration | Quantity | Price | Quantity & Availability | |||||
BP8003-1
|
10 U/μL | 7500 U | Item Discontinued This item has been discontinued by the supplier. Please Sign In to view product availability in your area. | N/A | |||||
BP8003-5
|
10 U/μL | 15,000 U | Item Discontinued This item has been discontinued by the supplier. Please Sign In to view product availability in your area. | N/A | |||||
Description
5'...G^A A T T C...3'
3'...C T T A A^G...5'
Conditions for 100% Activity:
- 1X OPTIZYME Buffer EcoRI: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl, 0.02% Triton X-100 and 0.1mg/ml BSA.
- Incubate at 37°C.
Enzyme Activity in OPTIZYME buffers:
- Buffer 1: 0 - 20%
- Buffer 2: No reaction
- Buffer 3: 100%
- Buffer 4: No reaction
- Buffer 5: 100%™
Storage Buffer:
10mM potassium phosphate (pH 7.4 at 25°C), 300mM NaCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 0.15% Triton X-100 and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with EcoRI.
Digestion of Agarose-embedded DNA:
A minimum of 5 units of EcoRI is required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Note: Low salt concentration, large excess of the enzyme, pH>8.0, or the replacement of mg2+ by Mn2+ may result in star activity.
Compatible Ends: XapI, MunI, TasI.
Methylation Effects:
Dam: never overlaps - no effect.
Dcm: never overlaps - no effect.
CpG: may overlap - cleavage impaired.
- 5'...m5C GAATTm5C G...3'
- 5'...TGm6AATTC(N)4TGCT...3'
Specifications
25 to 50% Water, >50% Glycerin, 2.5 to10% Sodium Chloride | |
7.4 | |
10mM Potassium Phosphate (pH 7.4 at 25°C), 300mM NaCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 0.15% Triton™ X-100, 50% Glycerol | |
G.AATTC | |
Liquid |
37°C | |
>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with EcoRI | |
Keep container tightly closed | |
EcoRI |
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