Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Learn More
OPTIZYME™ AloI, Fisher BioReagents™
Description
5'...^ 7(N) G A A C (N)6 T C C (N)12-13^...3'
3'...^12-13(N) C T T G (N)6 A G G (N)7 ^...5'
Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
- Incubate at 30°C
- Buffer 1: 0 - 20%
- Buffer 2: 0 - 20%
- Buffer 3: 0 - 20%
- Buffer 4: 20 - 50%
- Buffer 5: 100%
- 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol
- Incubation at 37°C results in 20% activity.
- AloI produces double-strand cuts on both sides from the interrupted recognition site. Its unique feature is a degenerate cleavage point on the 3 side of the recognition sequence (12 or 13 nucleotides away).
- The presence of S-adenosylmethionine in the reaction mixture results in incomplete cleavage with AloI.
- Greater than 10-fold overdigestion with AloI may result in star activity.
- AloI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.
Dam: May overlap - effect not determined
- 5'...GAAC(N)4Gm6A TCC...3'
- 5'...GAAC(N)6TCm5CW GG...3'
- 5'...m5C GAAC(N)6TCC...3'
- 5'...GAAC(N)6TCm5C G...3'
- 5'...GAAm5C G(N)5TCC...3'
Specifications
1 to 3 U/μL | |
30°C | |
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with AloI | |
Keep container tightly closed | |
(7/12)GAAC(N)6TCC(12/7) | |
Liquid |
25 to 50% Water, >50% Glycerin | |
7.4 | |
10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol | |
100 U | |
AloI |
Provide Content Correction
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Product Title
Spot an opportunity for improvement?
Provide Content Correction