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Description
5'...A T^C G A T...3'
3'...T A G C^T A...5'
Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 20 - 50%
- Buffer 2: 20 - 50%
- Buffer 3: 20 - 50%
- Buffer 4: 100%
- Buffer 5: 20 - 50%
- 10mM Tris-HCl (pH 8.0 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% (v/v) glycero
- ClaI is blocked by overlapping dam methylation. To avoid dam methylation, use a dam-, dcm- strain.
- 5'...ATCGm6A TC...3'
- 5'...ATCGAT...3'
Specifications
10 U/μL | |
37°C | |
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Cfr9I | |
Keep container tightly closed | |
AT.CGAT | |
Liquid |
>50% Water, 25 to 50% Glycerin | |
7.4 | |
10mM Tris HCl (pH 8 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol | |
600 U | |
ClaI (Bsu15I) |
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