Protein Purification Reagents and Kits
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Protein Purification Reagents and Kits
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Filtered Search Results
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Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Flow Rate | <20 mL/min. |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Diameter (English) Inner | 0.6 in. |
Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Diameter (Metric) Inner | 16 mm |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 3 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Invitrogen™ Ni-NTA Agarose
Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Type | Agarose |
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Stationary Phase | Ni-NTA |
Content And Storage | The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored. |
Form | Suspension |
Column Type | Affinity Column |
Cytiva HisTrap™ excel Prepacked Columns
For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Type | Pre-packed Column |
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Content And Storage | 4°C to 30°C |
Description | HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 2 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Cytiva PD MidiTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL. Cytiva Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Cytiva HisTrap™ FF Columns: 5 x 5mL
Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Cytiva PD MiniTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Cytiva Glutathione Sepharose™ 4B Media
Use for high capacity purification of Glutathione S-Transferase (GST) tagged fusion proteins. Cytiva Life Sciences™ Glutathione Sepharose™ 4B Media requires only a single-step for purification.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Particle Size | 90 μm |
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pH Range | 4 to 13 |
Material | Glutathione Sepharose 4 B |
Cytiva Ni Sepharose™ 6 Fast Flow
Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Particle Size | 90μm |
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Buffer | 0.1M HCl, 0.1M NaOH, 8M Urea |
Flow Rate | 600cm/hr. |
Height (Metric) | 250mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | Ni Sepharose 6 FF chromatography media |
dimMatrix | Ni-Sepharose |
Support Type | 6% Cross-linked Agarose |
Max. Pressure | 1 bar |
Height (English) | 9.84 in. |
Cytiva His MultiTrap™ 96-well Filter Plates
Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged proteins
Type | 96-well Filter Plate |
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Content And Storage | 4°C to 30°C |
Description | His MultiTrap FF and His MultiTrap HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC) |
pH Range | 3 to 12 |
Quantity | 4 x 96-well Filter Plates |
Material | Polypropylene, Polyethylene |
Thermo Scientific™ Pierce™ GST Spin Purification Kit, 1 mL
Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.
Cytiva HiScreen™ Sepharose™ Prepacked Fast Flow HIC Columns
Designed for method optimization and parameter screening
Particle Size | 45 to 165μm |
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Height (Metric) | 100mm |
Content And Storage | 4° to 30°C |
Diameter (English) Inner | 0.3 in. |
Bed Volume | 4.7mL |
pH Range | 3 to 13 |
Column Type | Ion Exchange Column |
Diameter (Metric) Inner | 7.7mm |
Quantity | 1 x 4.7mL |
Material | Polypropylene |
For Use With (Application) | Convenient process development optimization parameter screening |
Max. Pressure | 1.5 bar |
Height (English) | 3.93 in. |
Cytiva SOURCE™ Tricorn™ Columns for IEX
Source 15Q 4.6/100 PE is a strong anion exchanger prepacked in a Tricorn column for intermediate lab-scale purification and large-scale polishing of biomolecules with ion exchange chromatography.
Buffer | All common ion exchange Buffers |
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Height (Metric) | 100mm |
Content And Storage | 4° to 30°C |
Diameter (English) Inner | 0.18 in. |
Bed Volume | 1.7mL |
Column Type | Ion Exchange Column |
Diameter (Metric) Inner | 4.6mm |
Quantity | 1 x 1.7mL |
Material | PEEK |
For Use With (Application) | High performance ion exchange chromato graphy of Proteins, Peptides, Polynucleotides and other biomolecules |
Max. Pressure | 40 bar |
Particle Size | 15μm |
Flow Rate | <5mL/min. |
Support Type | Polystyrene/Divinylbenzene |
Height (English) | 3.93 in. |
Thermo Scientific™ HisPur™ Ni-NTA Resin
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Stationary Phase | Divalent Nickel, Metal Chelate |
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Type | Resin |
Target | His-Tagged Protein |
Content And Storage | Store at 4°C |
Format | Bottle |
Description | HisPur Ni-NTA Resin |
Form | Liquid Suspension |
Column Type | Affinity, Agarose Resin |
Capacity (Metric) | Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin |
Product Line | HisPur™ |