Thermo Scientific™ Pierce™ GST Spin Purification Kit, 1 mL

Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.

Supplier:  Thermo Scientific™ 16107

Catalog No. PI16107

Description

Thermo Scientific™ Pierce Glutathione Agarose is a high-capacity, high-performance resin for affinity purification of GST-tagged fusion proteins from cellular lysates.

The high–quality support consists of glutathione that has been immobilized by its central sulfhydryl group via a 12–atom spacer arm to crosslinked 6% beaded agarose. Purification of GST–fusion proteins using glutathione (GSH) agarose beads is well documented and adaptable to a variety of scales, column formats and specific applications. Whether the purpose is to purify large amounts of recombinant protein from over–expressing E. Coli lysates or to investigate protein interactions involving GST–tagged bait proteins, Pierce Glutathione Agarose is suitable for the task.

To accommodate these many uses, Pierce Glutathione Agarose is offered in several package sizes and formats. These include three volumes of resin slurry, three sizes of centrifuge-ready columns, complete GST purification kits, and two sizes of FPLC–ready chromatography cartridges.

Highlights:

• High capacity – binds at least 25mg of recombinant GST protein per milliliter of resin

• High yield and purity – consistently purifies at least 10mg of GST – tagged protein per milliliter of resin with greater than 90% purity

• Cost-effective – resin is economically priced and can be reused at least five times without reduction in binding capacity and purification performance

• Versatile – works well to purify GST – fusion proteins from bacterial lysates or use with pre-purified GST – tagged proteins to pull down protein interactions

• Compatible – validated and effective for use with Thermo Scientific Pierce Cell Lysis Reagents to extract and purify from bacterial or mammalian cell cultures

• Flexible – available in multiple formats including bulk resin, spin columns, chromatography cartridges and convenient GST purification kits

Available Formats:

• Resin Slurries: 10mL, 100mL, 500mL bottles

• Spin Columns: 0.2mL (microcentrifuge), 1mL, 3mL columns

• Purification Kits: complete kits in all three column sizes

• Chromatography Cartridges: 1mL and 5mL cartridges, compatible with automated liquid chromatography systems and with syringe processing

• Bulk and Custom: let us know your needs for bulk Glutathione Agarose

Includes:

Kits contain Pierce Glutathione Spin Columns, equilibration/wash buffer and glutathione to prepare elution buffer

Specifications

• Capacity (Metric): 1 mL
• Content And Storage: Sufficient For: Purifying approx. 10 mg of GST-tagged protein per column
  • Pierce Glutathione Spin Columns, 1 mL, 5 columns
  • Equilibration/Wash Buffer, 250 mL
  • Glutathione, 184 mg
  • Column Plugs, 5 plugs
  
  Store at 4°C.
• Quantity: 5 Column Kit
• Type: Spin Column Kit
• Sufficient For: 10 mg of GST-Tagged Protein per Column
• Column Type: Affinity, Agarose Resin

• Description: Pierce GST Spin Purification Kit, 1mL
• Product Line: Pierce™
• Target: GST-Tagged Protein
• Stationary Phase: Glutathione
• Format: Spin Column

For Research Use Only. Not for use in diagnostic procedures.