Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Catalog Number	Mfr. No.	Diameter (Metric) Inner	Quantity	Flow Rate
45-000-323	Cytiva 17524701	7 mm	5 x 1 mL	4 mL/min. (1 mL), 5 mL/min. (5 mL)
45-000-324	Cytiva 17524801	16 mm	1 x 5 mL	4 mL/min. (1 mL), 5 mL/min. (5 mL)
45-000-325	Cytiva 17524802	16 mm	5 x 5 mL	4 mL/min. (1 mL), 5 mL/min. (5 mL)
45-000-513	Cytiva 29051021	7 mm	1 mL	4 mL/min. (1 mL), 5 mL/min. (5 mL)

Description

• Prepacked with Ni Sepharose™ High Performance medium
• Negligible nickel ion leakage
• Pressure Max: 3 bar
• Column material: polypropylene
• 1 and 5mL columns
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
• Manual operation with syringe, pump or chromatography system

Media

• Matrix: 6% cross-linked agarose
• Average particle size: 34μm
• pH stability working range: 3 to 12

1mL columns

• Bed Dimensions: 7 x 25mm
• Flow velocity: 4mL/min.

5mL columns

• Bed Dimensions: 16 x 25mm
• Flow velocity: 5mL/min.

Specifications

• Description: HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
• Material: Polypropylene
• Support Type: 6% Highly Cross-linked Agarose
• Max. Operating Pressure: 5 bar (0.5 MPa, 70 psi)

• Flow Rate: 4 mL/min. (1 mL), 5 mL/min. (5 mL)
• pH Range: 3 to 12
• Content And Storage: 4°C to 30°C
• Type: Pre-packed Column