Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.

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Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Pricing & Availability
Specifications

Flow Rate	4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type	Ni Sepharose Pre-packed Column
Content And Storage	4°C to 30°C
Description	HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure	5 bar (0.5 MPa, 70 psi)
pH Range	3 to 12
Material	Polypropylene
Support Type	6% Highly Cross-linked Agarose

Cytiva Ni Sepharose™ 6 Fast Flow

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

Pricing & Availability
Specifications

Particle Size	90μm
Buffer	0.1M HCl, 0.1M NaOH, 8M Urea
Flow Rate	600cm/hr.
Height (Metric)	250mm
Content And Storage	4° to 30°C
pH Range	3 to 12
Product Type	Ni Sepharose 6 FF chromatography media
Support Type	6% Cross-linked Agarose
Max. Pressure	1 bar
Height (English)	9.84 in.

Cytiva PD MidiTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 M_r in sample volumes up to 1mL. Cytiva Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

Pricing & Availability

Cytiva Protein G Sepharose™ 4 Fast Flow Media

Rapid processing of large volumes, recoveries of 70 to 90%. Cytiva Protein G Sepharose™ 4 Fast Flow Media is recombinant protein G coupled to Sepharose 4 Fast Flow.

Pricing & Availability
Specifications

Particle Size	90 μm
Content And Storage	2°C to 8°C
Matrix	4% Cross Linked Agarose
Ligand	Recombinant Protein G lacking albumin binding region
Ligand Concentration	2mg Protein G/mL Medium
pH	3 to 9
For Use With (Application)	Used in a range of diagnostic and research applications

Cytiva HiTrap™ Heparin HP Columns

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

Pricing & Availability
Specifications

Particle Size	34 μm
Ligand Type	10mg Heparin/mL medium
Height (Metric)	2.5cm
Content And Storage	4° to 30°C
pH Range	5 to 10
Product Type	HiTrap Heparin Sepharose HP prepacked column
For Use With (Equipment)	Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Sample Type	DNA-binding Proteins, coagulation facto rs, Lipoproteins and Protein synthesis facto rs
Material	Polypropylene
For Use With (Application)	Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins
Support Type	6% Highly Cross-linked Agarose
Max. Pressure	42psi
Height (English)	0.98 in.

Cytiva HisTrap™ FF Columns: 16 × 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 × 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

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Pricing & Availability
Specifications

Flow Rate	<20 mL/min.
Type	Ni Sepharose Pre-packed Column
Content And Storage	4°C to 30°C, 20% Ethanol
Diameter (English) Inner	0.6 in.
Description	HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure	5 bar (0.5 MPa, 70 psi)
pH Range	3 to 12
Diameter (Metric) Inner	16 mm
Material	Polypropylene
Support Type	6% Cross-linked Agarose

Sartorius Vivapure™ AdenoPACK 20 Kit

The optimal kit for construct screening

Promotions are available

Pricing & Availability

Cytiva Protein A Sepharose™ CL-4B Medium

For immunoglobin purification and fractionation. Cytiva Protein A Sepharose™ CL-4B Medium is also well suited for immunoprecipitation.

Pricing & Availability
Specifications

Particle Size	90 μm
Content And Storage	2°C to 8°C
Matrix	4% Cross Linked Agarose
Ligand	Native Protein A
Ligand Concentration	3mg Protein A/mL Medium
Binding Capacity	20mg Human IgG/mL
pH	2 to 10
For Use With (Application)	For immunoglobin purification and fractionation and is suitable for immunoprecipitation procedures

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Protein Purification

Protein Purification

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