Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Catalog Number	Mfr. No.	Quantity
PI88221	Thermo Scientific™ 88221	10 mL
PI88222	Thermo Scientific™ 88222	100 mL
PI88223	Thermo Scientific™ 88223	500 mL

Description

Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin.

The specially prepared nickel-NTA support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.

HisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale.

Highlights:

• High capacity – bind up to 60mg of 6xHis-tagged protein per milliliter of resin
• Versatile – purify proteins using native or denaturing conditions
• Compatible – use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
• Flexible – available in multiple formats including bulk resin, spin columns and chromatography cartridges
• Cost-effective – reuse the same batch of resin at least five times
• Easy to use – pre-formulated buffers available for kit formats

Available Formats:

• Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
• Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
• Purification Kits – complete kits in all three column sizes
• Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
• Bulk and Custom – let us know your needs for bulk HisPur Ni-NTA Resin

Includes:

Kits contain HisPur Ni-NTA Columns (store at 4°C)

Specifications

• Capacity (Metric): Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin
• Content And Storage: Store at 4°C
• Product Line: HisPur™
• Type: Resin
• Format: Bottle

• Description: HisPur Ni-NTA Resin
• Form: Liquid Suspension
• Target: His-Tagged Protein
• Stationary Phase: Divalent Nickel, Metal Chelate
• Column Type: Affinity, Agarose Resin

For Research Use Only. Not for use in diagnostic procedures.