Competent Cells
Competent Cells
MilliporeSigma™ Novagen™ BL21 Competent Cells
BL21 host strain is the most widely used host background and has the advantage of being deficient in both lon and ompT proteases.
MilliporeSigma™ Novagen™ NovaBlue(DE3) Competent Cells
NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.
MilliporeSigma™ Novagen™ HMS174 (DE3) pLysS Competent Cells
HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. T7 lysozyme expression suppresses basal T7 expression.
MilliporeSigma™ Novagen™ HMS174 (DE3) Competent Cells
HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.
MilliporeSigma™ Novagen™ NovaBlue Singles™ Competent Cells
NovaBlue Singles Competent Cells are designed for ultimate convenience and reliability in plasmid transformation.
MilliporeSigma™ Novagen™ RosettaBlue™ (DE3)pLysS Competent Cells
RosettaBlue host strains combine high transformation efficiency with enhanced expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.
MilliporeSigma™ Novagen™ Rosetta-gami™ 2 Competent Cells
Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
MilliporeSigma™ Novagen™ Origami™ 2 Competent Cell Set
Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.
MilliporeSigma™ Novagen™ BL21 Competent Cell Set
BL21 is the most widely used host background and has the advantage of being deficient in both lon (1) and ompT proteases.
MilliporeSigma™ Novagen™ Tuner™ DE3 Competent Cells
Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.
MilliporeSigma™ Novagen™ Tuner™ Competent Cell Set
Tuner™ strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture.
MilliporeSigma™ Novagen™ Tuner™ DE3 pLysS Competent Cells
Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. T7 lysozyme expression suppresses basal T7 expression.
MilliporeSigma™ Novagen™ Origami™ B (DE3) pLacI Competent Cells
Origami B host strains are similar as the original Origami strain, except that they are derived from a lacZY mutant of BL21. Strain carries the pLacI plasmid producing extra Lac repressor.
MilliporeSigma™ Novagen™ Rosetta™ (DE3) pLacI Competent Cells
Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.
MilliporeSigma™ Novagen™ HT96™ BL21(DE3) Competent Cells
High-efficiency competent cells predispensed in 96-well plate for high-throughput applications
MilliporeSigma™ Novagen™ Rosetta-gami™ B (DE3) pLysS Competent Cells
Rosetta-gami B strains combines the key features of BL21, Origami, and Rosetta to enhances the expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.
MilliporeSigma™ Novagen™ Origami™ 2 (DE3) pLysS Competent Cells
Origami 2 strains have mutations in glutathione reducatase(gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation. T7 lysozyme expression suppresses basal T7 expression.
MilliporeSigma™ Novagen™ NovaBlue GigaSingles™ Competent Cells
NovaBlue GigaSingles™ Competent Cells produce greater than 1 × 10⁹ colonies/μg plasmid DNA for cloning applications requiring high-efficiency transformations.
MilliporeSigma™ Novagen™ Origami™ 2 (DE3) Competent Cells
Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.
MilliporeSigma™ Novagen™ Rosetta-gami™ 2 (DE3) pLacI Competent Cells
Rosetta-Gami 2 strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins. Contains the pLacI plasmid producing extra Lac repressor.
MilliporeSigma™ Novagen™ Origami™ 2 Competent Cells
Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.
MilliporeSigma™ Novagen™ Rosetta-gami™ 2 (DE3) Competent Cells
Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
MilliporeSigma™ Novagen™ Rosetta™ 2 (DE3) Competent Cells
Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.
MilliporeSigma™ Novagen™ BL21(DE3) Competent Cells
Achieve greater efficiency, reproducibility and convenience with Novagen prepared competent cells. BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high-level protein expression using a T7 RNA polymerase-IPTG induction system.
MilliporeSigma™ Novagen™ BL21(DE3) pLysS Competent Cells
BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.
MilliporeSigma™ Novagen™ NovaBlue Competent Cells
NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.
MilliporeSigma™ Novagen™ NovaBlue Competent Cells
NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.
Thermo Scientific™ DH5α Competent Cells; High Efficiency
High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors
Thermo Scientific™ DH10B Competent Cells; High Efficiency
High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.
Thermo Scientific™ DH5α Competent Cells
Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.