Competent Cells
Competent Cells
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Invitrogen™ One Shot™ Stbl3™ Chemically Competent E. coli
One Shot Stbl3 Chemically Competent E. coli are designed for cloning direct repeats found in lentiviral expression vectors as these cells reduce the frequency of homologous recombination of direct repeats found in lentiviral vectors (ViraPower Lentiviral Expression kits) and other retroviral vectors (instability-prone HIV-based plasmids).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ DH5α Competent Cells
Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.
Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli
One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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Blue/White Screening | Yes (lacZΔM15) |
Cloning Methylated DNA | Yes (mcrA) |
Transformation Efficiency Level | High Efficiency (>1 x 109 cfu/μg) |
High-throughput Compatibility | Low |
Improves Plasmid Quality | Yes (endA1) |
Preparing Unmethylated DNA | No |
Plasmid | High Copy Plasmid |
Reduces Recombination | Yes (recA1) |
T1 Phage - Resistant (tonA) | No |
Antibiotic Resistance Bacterial | Yes (Streptomycin) |
Format | Tube |
Product Type | Chemically Competent Cells |
Species | E. coli (K12) |
Product Line | One Shot™ |
Contains F' Episome | No |
Thermo Scientific™ DH5α Competent Cells; High Efficiency
High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors
Invitrogen™ MAX Efficiency™ DH5α Competent Cells
MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ BL21(DE3) Competent Cells
Suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.
Invitrogen™ One Shot™ ccdB Survival™ 2 T1R Competent Cells
One Shot ccdB Survival 2 T1R Competent Cells are suitable for the propagation of plasmids containing the ccdB gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ One Shot™ BL21(DE3)pLysS Chemically Competent E. coli
One Shot BL21(DE3)pLysS Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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Blue/White Screening | No |
Cloning Methylated DNA | No |
Transformation Efficiency Level | Medium Efficiency (1 x 108 to 1 x 109 cfu/μg) |
ToxicProteins | No |
High-throughput Compatibility | Low |
Improves Plasmid Quality | No |
ImprovesProteinStability | Yes (lon, ompT) |
Promoter | T7 |
Preparing Unmethylated DNA | Yes (dcm) |
Reduces Recombination | No |
ImprovesRNAStability | Yes (pLysS) |
T1 Phage - Resistant (tonA) | No |
Antibiotic Resistance Bacterial | Yes (Chloramphenicol) |
Format | Tube |
Product Type | Chemically Competent Cells |
Species | E. coli (B) |
Product Line | One Shot™ |
Contains F' Episome | No |
Invitrogen™ Subcloning Efficiency™ DH5α Competent Cells
Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 106 cfu/μg plasmid DNA.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ One Shot™ BL21 Star™ (DE3) Chemically Competent E. coli
One Shot BL21 Star (DE3) Chemically Competent E. coli are designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7-promoter-based expression systems such as Champion pET vectors.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ DH10B Competent Cells; High Efficiency
High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.
Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli
One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
---|---|
Blue/White Screening | Yes (lacZΔM15) |
Cloning Unstable DNA | No |
Cloning Methylated DNA | Yes (mcrA) |
Transformation Efficiency Level | High Efficiency (>1 x 1010 cfu/μg) |
High-throughput Compatibility | Low |
Improves Plasmid Quality | Yes (endA1) |
Preparing Unmethylated DNA | No |
Plasmid | May be used for plasmids >20 kb |
Reduces Recombination | Yes (recA13) |
T1 Phage - Resistant (tonA) | Yes (Streptomycin) |
Antibiotic Resistance Bacterial | No |
Format | Tube |
Product Type | Electrocompetent Cells |
Species | E. coli (K12) |
Product Line | One Shot™ |
Contains F' Episome | No |
Invitrogen™ One Shot™ BL21(DE3) Chemically Competent E. coli
One Shot BL21(DE3) Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ MultiShot™ StripWell TOP10 Chemically Competent E. coli
MultiShot StripWell TOP10 Chemically Competent E. coli cells are ideal for high-efficiency cloning and plasmid propagation, achieving a transformation efficiency of >1 x 108 cfu/μg with control plasmid DNA in StripWell format.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
---|---|
Blue/White Screening | Yes (lacZΔM15) |
Cloning Methylated DNA | Yes (mcrA) |
Transformation Efficiency Level | Medium Efficiency (1 x 108 to 1 x 109 cfu/μg) |
High-throughput Compatibility | High |
Improves Plasmid Quality | Yes (endA1) |
Preparing Unmethylated DNA | No |
Plasmid | High Copy Plasmid |
Reduces Recombination | Yes (recA1) |
T1 Phage - Resistant (tonA) | No |
Antibiotic Resistance Bacterial | Yes (Streptomycin) |
Product Type | Chemically Competent Cells |
Product Line | MultiShot™ |
Contains F' Episome | No |
MilliporeSigma™ Novagen™ Rosetta™ 2 Competent Cells
Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.
Content And Storage | ≤-70°C |
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Efficiency | Guaranteed efficiency: >2 x 106 cfu/μg |