Invitrogen™ Subcloning Efficiency™ DH5α Competent Cells

Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 10⁶ cfu/μg plasmid DNA.

Supplier:  Invitrogen™ 18265017

Catalog No. 18-265-017

Includes: Subcloning Efficiency DH5α Competent Cells: 4 vials, 500μL each (total of 2mL), pUC19 DNA (100pg/μL): 1 vial, 20μL

Description

Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 10⁶ cfu/μg plasmid DNA. Subcloning Efficiency DH5α Competent Cells are an economical solution for routine subcloning procedures or any general application where DNA for transformation is not limiting.

Features of Subcloning Efficiency DH5α Competent Cells
• Designed for general, low throughput, everyday use
• Standard laboratory strain useful for general cloning applications

Ideal for routine subcloning procedures
Subcloning Efficiency DH5α Competent Cells are recommended for routine subcloning of genes into plasmid vectors. 200 μL of chemically competent cells are pre-aliquoted in tubes for four reactions using 50 μL. These cells yield >1 x 10⁶ transformants/μg control DNA per 50 μL reaction. Subcloning Efficiency DH5α Competent Cells are not suitable for the generation of cDNA libraries.

Flexible cloning capabilities
Benefits of Subcloning Efficiency DH5α Competent Cells include:
• lacZΔM15 for blue/white color screening of colonies on plates containing X-gal or Bluo-gal
• recA1 that ensures increased insert stability and prevents unwanted recombination 
• endA1 that improves the yield and quality of plasmid DNA prepared from minipreps
• Supports replication of M13mp vectors but not plaque formation

Genotype
F^- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ-

Find the strain and format that you need
• We offer other DH strains in chemically competent and electrocompetent cell formats to meet your specific needs.
• Strains are available for challenging cloning applications such as library preparation or cloning unstable DNA.
• SOC medium (Cat. No. 15544034) for E. coli recovery after transformation is available, as well as other ready-to-use bacterial growth formats.

Order Info

Shipping Condition: Dry Ice

Specifications

• Product Type: Chemically Competent Cells
• Improves Plasmid Quality: Yes (endA1)
• Transformation Efficiency Level: Subcloning Efficiency (10⁶ to 10⁷ cfu/μg)
• Antibiotic Resistance Bacterial: No
• Blue/White Screening: Yes (lacZΔM15)
• Preparing Unmethylated DNA: No
• Shipping Condition: Dry Ice
• Species: E. coli (K12)
• Product Line: Subcloning Efficiency™

• Contains F' Episome: No
• Cloning Methylated DNA: No
• Content And Storage: • Subcloning Efficiency DH5α Competent Cells (4 x 500 μL)
  Store Competent Cells at –80°C.
  
  • pUC19 DNA (20 μL at 100 pg/μL)
  Store pUC19 DNA at –20°C.
  
  
• Cloning Unstable DNA: Not suitable for cloning unstable DNA
• High-throughput Compatibility: Low
• Reduces Recombination: Yes (recA1)
• T1 Phage - Resistant (tonA): No
• Format: Tube
• Quantity: 4 x 500 μL

For Research Use Only. Not for use in diagnostic procedures.