Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Learn More
Thermo Scientific™ DH10B Competent Cells, High Efficiency PROMO
High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.
Supplier: Thermo Scientific™ EC0113
Description
- Suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently
- These cells are ideal for the construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors
- The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal
- High transformation efficiency: >1x109 cfu/μg pUC19 DNA
- Suitable for routine and high-throughput cloning applications
- Allows for blue/white colony screening
- Convenient two reactions per vial packaging
- S.O.C. Outgrowth medium included
- Suitable for variety of applications requiring high transformation efficiency:
- Efficient DNA cloning both of prokaryotic and eukaryotic origin
- Cloning of large plasmids and BACs
- Ideal for generation of cDNA libraries using plasmid-derived vectors
- Site directed mutagenesis
- Genotype: F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ (ara-leu)7697 galU galK λ– rpsL(StrR) nupG
Note: Optimized for use with the CloneJET PCR Cloning Kit (Cat. Nos. K1231, K1232) and Phusion Site-Directed Mutagenesis Kit (Cat. No. F541), and available as combo versions (Cat. Nos. K123120, K123240, F542)
Specifications
Chemically Competent | |
Dry Ice (-80°C) | |
E. coli | |
Cloning |
Lacks F' Episome | |
DH10B | |
Tube | |
20 Reactions |
Provide Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Spot an opportunity for improvement?
Provide Content Correction