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Thermo Scientific™ DH5α Competent Cells

Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.

Supplier:  Thermo Scientific™ EC0111

Catalog No. FEREC0111

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  • The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gall
  • Transformation efficiency: >1x106 cfu/µg pUC19 DNA
  • Suitable for routine cloning applicationsConvenient economical packaging
  • Contain genetic markers that allow for blue/white colony screening
  • DH5α Competent Cells for Subcloning are suitable for the standard DNA cloning applications:
    • DNA cloning following PCR or restriction digestion reactions
    • Subcloning (transferring DNA from a parent vector to a destination vector)
    • Plasmid isolation
  • Genotype: F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1
  • For Research Use Only. Not for use in diagnostic procedures.


Chemically Competent
Dry Ice (-80°C)
E. coli
Lacks F' Episome
20 Reactions


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