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Thermo Scientific™ DH10B Competent Cells; High Efficiency

High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.

Supplier:  Thermo Scientific™ EC0113

Catalog No. FEREC0113


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Description

Description

  • Suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently
  • These cells are ideal for the construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors
  • The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal
  • High transformation efficiency: >1x109 cfu/µg pUC19 DNA
  • Suitable for routine and high-throughput cloning applications
  • Allows for blue/white colony screening
  • Convenient two reactions per vial packaging
  • S.O.C. Outgrowth medium included
  • Suitable for variety of applications requiring high transformation efficiency:
    • Efficient DNA cloning both of prokaryotic and eukaryotic origin
    • Cloning of large plasmids and BACs
    • Ideal for generation of cDNA libraries using plasmid-derived vectors
    • Site directed mutagenesis
  • Genotype: F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ (ara-leu)7697 galU galK λ– rpsL(StrR) nupG

Note: Optimized for use with the CloneJET PCR Cloning Kit (Cat. Nos. K1231, K1232) and Phusion Site-Directed Mutagenesis Kit (Cat. No. F541), and available as combo versions (Cat. Nos. K123120, K123240, F542)

Specifications

Specifications

Chemically Competent
Dry Ice (-80°C)
E. coli
Cloning
Lacks F' Episome
DH10B
Tube
20 Reactions
SDS
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Documents

Product Certifications
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