Invitrogen™ MAX Efficiency™ DH5α Competent Cells

MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors.

Supplier:  Invitrogen™ 18258012

Catalog No. 18-258-012

Includes: Max Efficiency DH5α Competent Cells: 5 vials, 200μL each (total of 1mL), pUC19 DNA (0.01μg/mL): 1 vial, 100μL, S.O.C. medium: 2 bottles, 6mL each

Description

MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α cells increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

Benefits of MAX Efficiency DH5α Competent Cells:
• Versatile strain for everyday cloning applications
• High quality plasmid preparations
• Useful for generating plasmid libraries 

DH5α competent cells for high transformation efficiency
The Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors, and can be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. DH5α cells are capable of being transformed efficiently with large plasmids, and can also serve as a host for the M13mp cloning vectors if a lawn of DH5αF′IQ (Cat. No. 18288019) (or similar) is provided to allow plaque formation.

Features of MAX Efficiency DH5α Competent Cells include:
• Transformation efficiency of >1 x 10⁹ transformants/μg plasmid DNA
• High plasmid yield from the DH5α (endA1) E. coli strain 
• Blue/white screening capable (lacZΔM15)
• Greater insert stability (recA1)

Genotype:
F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk-, mk+) phoA supE44 λ-thi-1 gyrA96 relA1 

Find the strain and format that fits you needs
• DH5α strain and its derivatives available in with a variety of transformation efficiencies and in both electrocompetent and chemically competent formats. 
• ElectroMAX™ DH5α-E Competent Cells (Cat. No. 11319019) are engineered for electroporation to achieve high transformation efficiency of >1 x 10¹⁰.
• DH5α T1R competent cells are available in MultiShot format for high throughput applications.

Order Info

Shipping Condition: Dry Ice

Specifications

• Product Type: Chemically Competent Cells
• Improves Plasmid Quality: Yes (endA1)
• Transformation Efficiency Level: High Efficiency (>1 x 10⁹ cfu/μg)
• Antibiotic Resistance Bacterial: No
• Blue/White Screening: Yes (lacZΔM15)
• Preparing Unmethylated DNA: No
• Shipping Condition: Dry Ice
• Species: E. coli (K12)
• Product Line: MAX Efficiency™

• Contains F' Episome: No
• Cloning Methylated DNA: No
• Content And Storage: • Max Efficiency DH5α Competent Cells (5 x 200 μL)
  Store Competent Cells at –80°C.
  
  • pUC19 DNA (100 μL at 0.01 μg/mL)
  Store pUC19 DNA at –20°C.
  
  • S.O.C. Medium (2 x 6 mL)
  Store S.O.C. Medium at 4°C or room temperature.
  
  
• Cloning Unstable DNA: Not suitable for cloning unstable DNA
• High-throughput Compatibility: Low
• Reduces Recombination: Yes (recA1)
• T1 Phage - Resistant (tonA): No
• Format: Tube
• Quantity: 5 x 200 μL

For Research Use Only. Not for use in diagnostic procedures.