Direct PCR Reagents and Kits
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Direct PCR Reagents and Kits
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Thermo Scientific™ Phire Plant Direct PCR Master Mix
Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.
Shipping Condition | Wet or Dry Ice |
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Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Master Mix |
Sample Type | Plant |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Genotyping,Hot-start PCR |
PCR Method | Direct PCR |
Invitrogen™ Platinum™ Direct PCR Universal Master Mix
Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.
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Shipping Condition | Dry Ice |
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Polymerase | Platinum II Taq Hot-Start DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 1X |
Size (Final Product) | 8 kb |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Universal Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Green Features | Less Hazardous, Less Waste |
Concentration | 2X |
For Use With (Application) | Hot-start PCR,Real Time PCR (qPCR) |
Thermo Scientific™ Phire Animal Tissue Direct PCR Kit (without sampling tools)
Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects.
Invitrogen™ PureLink™ PCR Purification Kit
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
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Shipping Condition | Room Temperature |
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Format | Kit |
Sample Type | PCR Products |
Isolation Technology | Silica Spin Column |
Run Time | 10 min. |
Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
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GC-Rich PCR Performance | High |
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Format | Tube |
Product Type | 1-Step qPCR kit |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Sample Type | Cell, RNA |
Reaction Speed | Fast or Standard |
Reagent Type | Cell Lysis |
Green Features | Fewer resources used and less waste, Less hazardous |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Product Line | Cells-to-CT™, TaqMan™ |
Detection Method | Primer-probe |
Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)
Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.
Thermo Scientific™ Phusion Blood Direct PCR Kit
Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
Shipping Condition | Wet or Dry Ice |
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Polymerase | Phusion Blood II DNA Polymerase |
GC-Rich PCR Performance | High |
Reaction Format | Separate Components |
Product Type | Direct PCR Kit |
Sample Type | Blood |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2 U/μL |
Starting Material | DNA |
PCR Method | Direct PCR |
Invitrogen™ Cells-to-cDNA™ II Kit
The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.
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Shipping Condition | Dry Ice |
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Content And Storage | 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at -20°C. Nuclease-free water may be stored at any temperature. |
Reverse Transcriptase | M-MLV |
Size (Final Product) | 7 kb or less |
Final Product Type | cDNA |
Sample Type | Mammalian Cells |
Purification Time | 2 hr. |
Optimal Reaction Temperature | 42°C |
For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Product Line | Ambion™, Cells-to-cDNA™ |
Applied Biosystems™ MagMAX™ Viral/Pathogen Binding Beads
These binding beads are included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit.
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Thermo Scientific™ GeneJET PCR Purification Kit
Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
Format | Spin Column |
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Run Time | 5 min. |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit
ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.
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Shipping Condition | Room Temperature |
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Target | DNA from Enzymatic Reactions (e.g. PCR) |
Content And Storage | The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly. |
Format | Kit |
Product Type | PCR Clean-Up Kit |
Sample Type | PCR Products |
Isolation Technology | ChargeSwitch™ Chemistry |
Run Time | 5 min |
For Use With (Application) | Next-Generation Sequencing (NGS),Cloning,Standard PCR |
High-throughput Compatibility | High-throughput Compatible |
Product Line | ChargeSwitch™ |
Thermo Scientific™ GeneJET PCR Purification Kit PROMO
GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
Format | Spin Column |
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Run Time | 5 min. |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit
Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
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GC-Rich PCR Performance | High |
---|---|
Format | Tube |
Product Type | 1-Step qPCR kit |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Sample Type | Cell, RNA |
Reaction Speed | Standard |
Reagent Type | Cell Lysis |
Green Features | Fewer resources used and less waste, less hazardous |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Product Line | Cells-to-CT™, Power SYBR™ Green |
Detection Method | SYBR |
Thermo Scientific™ Phire Tissue Direct PCR Master Mix
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
Shipping Condition | Wet or Dry Ice |
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GC-Rich PCR Performance | Medium |
Includes Label or Dye | Yes |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Genotyping,Hot-start PCR |
PCR Method | Direct PCR |
Polymerase | Phire Hot Start II DNA Polymerase |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Master Mix |
Sample Type | Tissue |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Starting Material | DNA |
Invitrogen™ PureLink™ PCR Micro Kit
PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.
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Shipping Condition | Room Temperature |
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Separation Range | 100 bp to 12 kb |
Content And Storage | Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature. |
Starting Material Volume | 125 μL |
Isolation Technology | Silica Spin Column |
Elution Volume Max | 20 μL |
For Use With (Application) | Cloning,Standard PCR |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Target | DNA from Enzymatic Reactions (e.g. PCR) |
Format | Spin Column |
Product Type | PCR Micro Kit |
Run Time | 10 min. |
Elution Volume Min | 5 μL |
Product Line | PureLink™ |