Thermo Scientific™ Phire Animal Tissue Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects.

Supplier:  Thermo Scientific™ F140WH

Catalog No. F140WH

Description

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects. The kit contains all the necessary components for amplification of DNA directly from animal tissues: optimized PCR reagents, Dilution buffer and DNARelease Additive, which can be used to improve the release of DNA from animal tissues. In addition, the kit includes control primers for positive control reactions that are universal and work with numerous animal species. A detailed manual is provided that describes protocols when working with different types of tissues.

Highlights

• No need for time-consuming and expensive DNA purification steps
• Very little sample material required
• Two simple protocols for various applications
• Phire Hot Start II DNA Polymerase (Cat. No. F122L) provides high yields of specific product with short extension time (20 s/kb).

The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq-based polymerases.

For optimal results start by accurately calculating your Tm with our Tm calculator.

Specifications

• Content And Storage: • Phire Hot Start II DNA Polymerase, 200 μL
  • 2X Phire Animal Tissue PCR Buffer, 5 x 1.25 mL
  • Control Primer Mix (25 μM each), 40 μL
  • Dilution Buffer, 2 x 5 mL
  • DNARelease Additive, 3 x 100 μL
  • Gel Loading Dye, 3 x 1 mL
  
  Store at -20°C.
• PCR Method: Direct PCR
• Reaction Speed: Fast
• Shipping Condition: Wet or Dry Ice
• No. of Reactions: 500 Reactions
• Reaction Format: Separate Components
• Starting Material: DNA

• GC-Rich PCR Performance: Medium
• Polymerase: Phire Hot Start II DNA Polymerase
• Product Type: Direct PCR Kit
• Hot Start: Built-In Hot Start
• Overhang: Blunt
• Sample Type: Tissue

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.