Thermo Scientific™ Phusion Blood Direct PCR Kit

Perfrom PCR directly from whole blood without DNA purification.

Catalog Number	Mfr. No.	Quantity
F547S	Thermo Scientific™ F547S	100 x 20 μL Reactions
F547L	Thermo Scientific™ F547L	500 Reactions

Description

Phusion Blood Direct PCR Kit is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to PCR inhibitors present in blood and retains its activity even with samples containing 40% whole blood.

This extremely accurate proofreading DNA polymerase makes the Phusion Blood Direct PCR Kit an ideal choice even for demanding applications, such as cloning. Phusion Blood Direct PCR Kit also allows analysis of blood samples with different anticoagulants, storage conditions and from various species.

Highlights

• No need for time-consuming and expensive DNA purification steps
• Simple protocol requires minimal hands-on time
• Extremely short PCR protocol times
• Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product

Specifications

• GC-Rich PCR Performance: High
• Polymerase: Phusion Blood II DNA Polymerase
• Product Line: Phusion
• Hot Start: Built-In Hot Start
• Reaction Format: Separate Components
• Starting Material: DNA

• PCR Method: Direct PCR
• Reaction Speed: Fast
• Product Type: Direct PCR Kit
• Overhang: Blunt
• Sample Type: Blood

For Research Use Only. Not for use in diagnostic procedures.