Standard PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.

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Invitrogen™ Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System

Platinum Quantitative RT-PCR ThermoScript One-Step System is for real-time quantitation of RNA molecules from total or poly(A)+ RNA in a single step.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Taq DNA Polymerase
Content And Storage	Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System contains ThermoScript™ RT/Platinum™ Taq mix, and 2X ThermoScript™ reaction mix (a buffer containing 0.4 mM of each dNTP, 6 mM MgSO₄ ). A vial of 50 mM MgSO₄ is also provided. Store at -20°C. Guaranteed stable for 6 months when properly stored.
Product Type	RT-PCR One-Step System
Sample Type	RNA
Reaction Speed	Standard
PCR Method	1-step RT-qPCR
Product Line	Platinum™, ThermoScript™
Detection Method	Primer-probe

Invitrogen™ SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.

Promotions are available

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Taq DNA Polymerase
Product Type	One-Step qRT-PCR Kit
For Use With (Equipment)	7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Sample Type	RNA
Reaction Speed	Standard
For Use With (Application)	Real Time PCR (qPCR)
PCR Method	1-step RT-qPCR
Product Line	Platinum™, SYBR™, SuperScript™
Detection Method	SYBR

Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit w/ROX

One-Step qRT-PCR Kits with ROX combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Platinum Taq DNA Polymerase
For Use With (Equipment)	7000 System, 7300 System, 7700 System, 7900HT System
Sample Type	RNA
Reaction Speed	Standard
For Use With (Application)	Gene Expression,Pathogen Detection,Virus Detection
PCR Method	1-step RT-qPCR
Product Line	Platinum™, SuperScript™
Detection Method	Primer-probe

Invitrogen™ EXPRESS One-Step Superscript™ qRT-PCR Kit, with premixed ROX

Next-generation, real-time qRT-PCR reagents designed to complement fast-cycling instruments. Get the turnaround time you want with the data quality you need.

Pricing & Availability
Specifications

Polymerase	Taq DNA Polymerase
Content And Storage	EXPRESS One-Step SuperScript™ qRT-PCR SuperMixes are composed of 2 modules: an EXPRESS RT module (containing SuperScript™ III Reverse Transcriptase and RNAseOUT™) and an EXPRESS qPCR SuperMix module with ROX premixed or in a separate tube. Store at -20°C upon receipt. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed stable for 6 months when properly stored.
Format	Tube
Product Type	qRT-PCR Kit
For Use With (Equipment)	7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, StepOne™, Fast Mode, StepOne™, Standard Mode
Sample Type	RNA
Reaction Speed	Fast, Standard
For Use With (Application)	Gene Expression
PCR Method	1-step RT-qPCR
Product Line	SuperScript™
Detection Method	Primer-probe

Invitrogen™ EXPRESS One-Step SYBR™ GreenER™ Kit, universal

EXPRESS One-Step SYBR GreenER kits are compatible with fast-cycling instruments for your SYBR-based qPCR experiments.

Promotions are available

Pricing & Availability
Specifications

Polymerase	Taq DNA Polymerase
Content And Storage	EXPRESS One-Step SYBR™ GreenER™ SuperMix Kits are composed of 2 modules: EXPRESS SYBR™ GreenER™ RT module (containing SuperScript™ III Reverse Transcriptase and RNAseOUT™) and EXPRESS SYBR™ GreenER™ qPCR SuperMix with ROX in a separate tube. Store at -20°C upon receipt. Once thawed, the EXPRESS SYBR™ GreenER™ qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed to be stable for 6 months when properly stored.
Format	Tube
Product Type	One-Step qRT-PCR Kit
For Use With (Equipment)	7500 Fast System, 7500 System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, BioRad CFX96™, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ
Sample Type	RNA
Reaction Speed	Fast, Standard
PCR Method	1-step RT-qPCR
Product Line	SYBR™ GreenER™
Detection Method	SYBR

Invitrogen™ EXPRESS One-Step SYBR™ GreenER™ Kit, with premixed ROX

Promotions are available

Pricing & Availability
Specifications

Polymerase	Taq DNA Polymerase
Content And Storage	EXPRESS One-Step SYBR™ GreenER™ SuperMix Kits are composed of 2 modules: EXPRESS SYBR™ GreenER™ RT module (containing SuperScript™ III Reverse Transcriptase and RNAseOUT™) and EXPRESS SYBR™ GreenER™ qPCR SuperMix with ROX premixed. Store at -20°C upon receipt. Once thawed, the EXPRESS SYBR™ GreenER™ qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed to be stable for 6 months when properly stored.
Format	Tube
For Use With (Equipment)	7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, StepOne™, Fast Mode, StepOne™, Standard Mode
Sample Type	RNA
Reaction Speed	Fast, Standard
For Use With (Application)	Gene Expression
PCR Method	1-step RT-qPCR
Product Line	SYBR™ GreenER™
Detection Method	SYBR

Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents

Promotions are available

Pricing & Availability
Specifications

Content And Storage	RT
Purity or Quality Grade	Molecular Biology
Form	Liquid
pH	7
Chemical Name or Material	Saline-Sodium Citrate
DNase	DNase free
Concentration	2.9 to 3.1M

Cytiva PuReTaq Ready-To-Go™ PCR Beads

Pre-mixed, pre-dispensed reaction beads optimized for performing standard PCR amplifications. Cytiva Lifescience™ illustra™ PuReTaq Ready-To-Go™ PCR Beads are stable at ambient temperature.

Promotions are available

Pricing & Availability
Specifications

Content And Storage	Room temperature stable beads containing ∼ 2.5 units of recombinant PuReTaq DNA Polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction Buffer, When a bead is reconstituted to a 25μL final volume, the concentration of each dNTP
Quantity	1 Kit
For Use With (Application)	Standard PCR

Cytiva Hot Start Mix RTG

Premixed, predispensed, ambient temperature-stable formulation providing robust and reliable performance for demanding PCR applications in which high specificity and high sensitivity are essential to success

Pricing & Availability
Specifications

Content And Storage	1.75 units of recombinant PuReTaq DNA Polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction Buffer. Ambient temperature in pouch with desiccant
Format	Tube
Product Type	illustra Hot Start Mix RTG
For Use With (Application)	Standard PCR

Thermo Scientific™ Taq DNA Polymerase, native, with BSA (5 U/µL)

Optimize routine PCR with this highly thermostable, native DNA polymerase.

Pricing & Availability

Thermo Scientific™ Taq DNA Polymerase, native, without BSA (5 U/µL)

Optimize amplification of DNA samples of low purity with this highly thermostable, native DNA polymerase for routine PCR.

Pricing & Availability

Thermo Scientific Chemicals Guanidine thiocyanate, 99+%, for molecular biology

Pricing & Availability

Thermo Scientific™ Phire Animal Tissue Direct PCR Kit (without sampling tools)

PCR directly from various animal tissues including mice, fish, birds and insects without DNA purification.

Pricing & Availability

Thermo Scientific™ Phire Hot Start II DNA Polymerase

Optimize routine and high throughput PCR applications requiring speed and specifity with this novel hot start DNA polymerase.

Pricing & Availability
Specifications

Polymerase	Phire Hot Start II DNA polymerase
GC-Rich PCR Performance	Medium
Fidelity (vs. Taq)	2 X
Purity or Quality Grade	DHPLC
Reaction Format	Standalone
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	50X
Starting Material	DNA
For Use With (Application)	Cloning
Product Line	Phire

Thermo Scientific™ Phusion Blood Direct PCR Master Mix

Amplify DNA directly from whole blood without prior purification

Pricing & Availability
Specifications

Polymerase	Phusion Blood II DNA Polymerase
GC-Rich PCR Performance	High
Includes Label or Dye	Yes
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Blood
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Starting Material	DNA
PCR Method	Direct PCR
Product Line	Phusion

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Standard PCR Products

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