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Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Content And Storage | • Phusion DNA Polymerase (2 U/μL) • 5X Phusion HF & GC Buffers • DMSO • 50 mM MgCl2 solution Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration. Store at -20°C. |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | Standalone |
Product Type | High-Fidelity DNA Polymerase |
Reaction Speed | Fast |
Hot Start | No |
Overhang | Blunt |
Concentration | 2 U/μL |
For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific™ DreamTaq Green PCR Master Mix (2X)
Save time in PCR applications with Master Mix and get higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase.
Polymerase | DreamTaq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1 X |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
Product Line | DreamTaq™ |
Cytiva PuReTaq Ready-To-Go™ PCR Beads
Pre-mixed, pre-dispensed reaction beads optimized for performing standard PCR amplifications. Cytiva Lifescience™ illustra™ PuReTaq Ready-To-Go™ PCR Beads are stable at ambient temperature.
Content And Storage | Room temperature stable beads containing ∼ 2.5 units of recombinant PuReTaq DNA Polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction Buffer, When a bead is reconstituted to a 25μL final volume, the concentration of each dNTP |
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Quantity | 1 Kit |
For Use With (Application) | Standard PCR |
Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF Buffer
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.
Shipping Condition | Dry Ice |
---|---|
Polymerase | Phusion High-Fidelity DNA Polymerase |
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | High-Fidelity PCR Master Mix |
Reaction Speed | Fast |
Hot Start | No |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific™ Taq DNA Polymerase, recombinant (5 U/μL)
Optimize routine PCR with this highly thermostable DNA polymerase.
Thermo Scientific™ PCR Master Mix (2X)
Optimize routin PCR with this highly thermostable DNA polymerase.
Polymerase | Taq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1 X |
Reaction Format | SuperMix or Master Mix |
Product Type | PCR Master Mix (2X) |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
Thermo Scientific™ DreamTaq PCR Master Mix (2X)
Get higher sensitivity, longer PCR products and higher yields in all standard PCR applications with this enhanced Taq DNA polymerase.
Polymerase | DreamTaq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1 X |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
Product Line | DreamTaq™ |
Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit
Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.
Shipping Condition | Dry Ice |
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Content And Storage | • RevertAid Reverse Transcriptase |
Reverse Transcriptase | RevertAid |
Final Product Type | First-Strand cDNA |
For Use With (Application) | RT-PCR |
Format | Kit |
Size (Final Product) | Up to 13 kb |
Reaction Format | Separate components |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix
Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.
Shipping Condition | Dry Ice |
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Polymerase | Phusion Flash II DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 25X |
Size (Final Product) | 20 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | High-Fidelity PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Taq DNA Polymerase, recombinant (1 U/μL)
Optimize routine PCR with this highly thermostable DNA polymerase.
Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR
The Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR, available with or without dsDNase, is a convenient system optimized for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | Maxima |
Final Product Type | First-Strand cDNA |
For Use With (Application) | Real Time PCR (qPCR) |
Format | Kit |
Size (Final Product) | Up to 20 kb |
Reaction Format | Separate components |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 50°C to 55°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ DreamTaq Green DNA Polymerase (5 U/μL)
Save time in standard PCR applications with this enhanced Taq DNA polymerase, supplied with colored buffer enabling direct gel loading.
Thermo Scientific™ Maxima SYBR Green/ROX qPCR Master Mix (2X)
Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.
Polymerase | Taq DNA Polymerase |
---|---|
GC-Rich PCR Performance | High |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
For Use With (Equipment) | 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System |
Sample Type | DNA, dsDNA |
Reaction Speed | Standard |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | Maxima |
Detection Method | SYBR |
Thermo Scientific™ Phusion™ Hot Start II DNA Polymerase (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.
Shipping Condition | Dry Ice |
---|---|
Polymerase | Phusion Hot Start II DNA Polymerase |
GC-Rich PCR Performance | High |
Content And Storage | • Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/μL) • 5X Phusion HF & GC Buffers • DMSO • 50 mM MgCl2 solution Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration. Store at -20°C. |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | Standalone |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2 U/μL |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)
Get higher sensitivity, longer PCR products and higher yields in all standard PCR applications with this enhanced Taq DNA polymerase.
Polymerase | DreamTaq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1 X |
Reaction Format | Standalone |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
Product Line | DreamTaq™ |