Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Perform short PCR protocols without compromising fidelity or yield with this master mix.

Catalog Number	Mfr. No.	Quantity
F548S	Thermo Scientific™ F548S	100 Reactions
F548L	Thermo Scientific™ F548L	500 Reactions

Description

The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases.

For optimal results start by accurately calculating your Tm with our Tm calculator.

Phusion Flash PCR Master Mix was developed to save valuable laboratory time. Ready-to-use 2X master mix enables the use of extremely short PCR protocols without compromising either the fidelity or the yield in the reaction. In addition, pipetting steps are minimized: only template and primers need to be added by the user.

The unique composition of the master mix enables the use of extremely short cycling protocols with both low and high complexity DNA templates - 15 seconds per kilobase or less. The master mix utilizes Phusion Flash II DNA Polymerase, a proofreading DNA polymerase modified from Phusion Hot Start II High-Fidelity DNA Polymerase.

Highlights

• Extreme speed: extension times of 15 s/kb or less
• Hot start modification allowing “zero-time reactivation”
• Accuracy: proofreading DNA polymerase with a fidelity of 25X Taq
• High yields in reduced time

Specifications

• Format: Tube
• Polymerase: Phusion Flash II DNA Polymerase
• Product Line: Phusion
• Concentration: 2X
• Hot Start: Built-In Hot Start
• Reaction Format: SuperMix or Master Mix
• Starting Material: DNA

• GC-Rich PCR Performance: High
• Reaction Speed: Fast
• Product Type: High-Fidelity PCR Master Mix
• Fidelity (vs. Taq): 25 X
• Overhang: Blunt
• Size (Final Product): 20 kb or Less

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppels. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.