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Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix
Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.
$121.00 - $535.00
Specifications
| GC-Rich PCR Performance | High |
|---|---|
| Polymerase | Phusion Flash II DNA Polymerase |
| Reaction Speed | Fast |
| Product Type | High-Fidelity PCR Master Mix |
| Shipping Condition | Dry Ice |
Description
Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time. Ready-to-use 2X master mix preserves the fidelity and the yield in the reaction when using extremely short PCR protocols. Additionally, the user only needs to add template and primers minimizing the number of pipetting steps.
The unique master mix composition permits usage of extremely short cycling protocols with both low and high complexity DNA templates - 15 seconds per kilobase or less. The master mix utilizes Phusion Flash II DNA Polymerase, a modified proofreading DNA polymerase derived from Phusion Hot Start II High-Fidelity DNA Polymerase.
Features
• Extreme speed: extension times of 15 s/kb or less
• Hot start modification allowing 'zero-time reactivation'
• Accuracy: proofreading DNA polymerase with a fidelity of 25X Taq
• High yields in reduced time
Applications
• Fast PCR
• High-fidelity PCR
• Difficult (GC-rich) templates
• Template generation for sequencing
• Multiplex PCR
• Long-range PCR
• Cloning
• Mutagenesis
• Microarray
Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.
Specifications
| High | |
| Fast | |
| Dry Ice | |
| 2X | |
| Built-In Hot Start | |
| SuperMix or Master Mix |
| Phusion Flash II DNA Polymerase | |
| High-Fidelity PCR Master Mix | |
| Hot-start PCR, High-fidelity PCR | |
| 25X | |
| Blunt | |
| 20 kb or less |
For Research Use Only. Not for use in diagnostic procedures.
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