Thermo Scientific™ PCR Master Mix (2X)

Optimize routin PCR with this highly thermostable DNA polymerase.

Catalog Number	Mfr. No.	Product Type	Quantity
FERK0171	Thermo Scientific™ K0171	PCR Master Mix (2X)	200 Reactions
FERK0172	Thermo Scientific™ K0172	PCR Master Mix (2X)	1000 Reactions

Description

A 950 bp single copy gene from human genomic DNA was amplified at a wide range of Mg²⁺ concentrations.
M - GeneRuler 100 bp Plus DNA Ladder

Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.

PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.

Highlights

• Thermostable -half life is more than 40 min at 95°C
• Generates PCR products with 3'-dA overhangs
• Supplied with two buffers -10X Taq Buffer with KCl and 10X Taq Buffer with (NH₄)₂SO₄. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)

Specifications

• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Fidelity (vs. Taq): 1 X
• Overhang: 3'-A

• Polymerase: Taq DNA Polymerase
• Product Type: PCR Master Mix (2X)
• Hot Start: No
• Reaction Format: SuperMix or Master Mix

For Research Use Only. Not for use in diagnostic procedures.