Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.

Catalog Number	Mfr. No.	No. of Reactions
F530S	Thermo Scientific™ F530S	100 Reactions
F530L	Thermo Scientific™ F530L	500 Reactions
F530XL	Thermo Scientific™ F530XL	2000 Reactions

Description

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

Features
• High fidelity (52X Taq)
• Fast PCR due to short extension times (15–30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications
• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.

Specifications

• Content And Storage: • Phusion DNA Polymerase (2 U/μL)
  • 5X Phusion HF & GC Buffers
  • DMSO
  • 50 mM MgCl₂ solution
  
  Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl₂ in the final 1X concentration.
  Store at -20°C.
• Reaction Speed: Fast
• Shipping Condition: Dry Ice
• Concentration: 2 U/μL
• Hot Start: No
• Reaction Format: Standalone

• GC-Rich PCR Performance: High
• Product Type: High-Fidelity DNA Polymerase
• For Use With (Application): Standard PCR, High-fidelity PCR
• Fidelity (vs. Taq): 52X
• Overhang: Blunt
• Size (Final Product): 20 kb or less

For Research Use Only. Not for use in diagnostic procedures.