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Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged proteins
Type | 96-well Filter Plate |
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Content And Storage | 4°C to 30°C |
Description | His MultiTrap FF and His MultiTrap HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC) |
pH Range | 3 to 12 |
Quantity | 4 x 96-well Filter Plates |
Material | Polypropylene, Polyethylene |
Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments
Content And Storage | 1 Vial, Store at 2°C to 8°C |
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Sufficient For | 10 mL Solution |
Thermo Scientific™ Pierce Protease and Phosphatase Inhibitor Mini Tablets broad-spectrum protease and pghospahatase inhibitor formulation provides complete protection from dephosphorylation and proteolytic degradation.
Content And Storage | 1 Vial, Store at 2°C to 8°C |
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Sufficient For | 10 mL Solution |
Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL. Cytiva Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Use for buffer exchange and cleanup of biological samples, for example peptides, small proteins, and oligosaccharides. Cytiva Life Sciences™ PD MiniTrap™ G-10 Sample Preparation Columns are prepacked, single-use gravity columns.
Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Particle Size | 90μm |
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Buffer | 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol |
Flow Rate | 150 to 600cm/hr. |
Content And Storage | 4° to 30°C |
pH Range | 2 to 12 |
Product Type | Ni Sepharose excel chromato graphy media |
dimMatrix | Ni-Sepharose |
Support Type | 6% Highly Cross-linked Agarose |
Use for coupling antibodies or other large proteins containing -NH2 groups to the Sepharose media. CNBr-Activated Sepharose™ Medium is a pre-activated media perfect for custom affinity applications.
Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium.
The DynaMag-2 magnet combines a strong magnetic attraction for efficient separations in standard microcentrifuge tubes with a flexible and smart ergonomic design.
Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.
Particle Size | 34 μm |
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Ligand Type | 10mg Heparin/mL medium |
Height (Metric) | 2.5cm |
Content And Storage | 4° to 30°C |
pH Range | 5 to 10 |
Product Type | Heparin HP Column |
For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
Sample Type | DNA-binding Proteins, coagulation facto rs, Lipoproteins and Protein synthesis facto rs |
Material | Polypropylene |
For Use With (Application) | Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins |
Support Type | 6% Highly Cross-linked Agarose |
Max. Pressure | 42psi |
Height (English) | 0.98 in. |
Use for high capacity purification of Glutathione S-Transferase (GST) tagged fusion proteins. Cytiva Life Sciences™ Glutathione Sepharose™ 4B Media requires only a single-step for purification.
Particle Size | 90 μm |
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pH Range | 4 to 13 |
Material | Glutathione Sepharose 4 B |
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
Flow Rate | <20 mL/min. |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Diameter (English) Inner | 0.6 in. |
Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Diameter (Metric) Inner | 16 mm |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Use for high-recovery desalting, buffer exchange and removal of low-molecular weight compounds packed with Sephadex™ G-25 Medium. Cytiva Life Sciences™ LabMate™ PD-10 Buffer Reservoirs provide fast, affordable performance.