Gel Filtration Media and Columns

A variety of columns and media for the size-based separation and purification of proteins, lipoproteins, peptides, etc. using gel filtration chromatography techniques.

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Cytiva HiTrap™ Heparin HP Columns

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

Pricing & Availability
Specifications

Particle Size	34 μm
Ligand Type	10mg Heparin/mL medium
Height (Metric)	2.5cm
Content And Storage	4° to 30°C
pH Range	5 to 10
Product Type	HiTrap Heparin Sepharose HP prepacked column
For Use With (Equipment)	Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Sample Type	DNA-binding Proteins, coagulation facto rs, Lipoproteins and Protein synthesis facto rs
Material	Polypropylene
For Use With (Application)	Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins
Support Type	6% Highly Cross-linked Agarose
Max. Pressure	42psi
Height (English)	0.98 in.

Cytiva Sephadex™ LH-20

Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Cytiva Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species.

Pricing & Availability
Specifications

Particle Size	18 to 111 μm in dry form
Height (Metric)	30cm
Flow Rate	720 cm/hr.
Content And Storage	4 to 30°C
Format	Solid Spherical Bead
pH Range	2 to 13
Product Type	Sephadex LH-20
Sample Type	Steroid, Terpenoid, Lipid, low molecular weight Peptide
Diameter (Metric) Inner	2.6cm
For Use With (Application)	For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures
Support Type	Hydroxypropylated, cross-linked Dextran
Max. Pressure	25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.
Height (English)	11.81 in.

Cytiva Superdex 30 Increase Precision 3.2/300 GL Prepacked SEC Column

Designed for high resolution in small-scale preparative purification and analysis for sample volumes up to 500μL

Pricing & Availability

Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids

Pricing & Availability
Specifications

Particle Size	25 to 75 μm
Height (Metric)	60cm
pH Range	3 to 11
For Use With (Equipment)	Low to medium pressure AKTA™ systems or pumped chromatography systems
Sample Type	Polysaccharides, macromolecules with extended structures and even small plasmids
Quantity	Each
Material	Polypropylene
For Use With (Application)	Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
Support Type	Allyl Dextran and N,N'-methylenebisacrylamide
Max. Pressure	1.5 bar [0.15 MPa] (22 psi)
Height (English)	23.62 in.

Cytiva Sephacryl™ S-300 High Resolution Media

Offer good recovery and low nonspecific adsorption. Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules. Enable gel filtration at laboratory and industrial scale, and may be purchased in pre-packed columns.

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Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

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Cytiva Superdex 30 PG size exclusion chromatography media prepacked into HiLoad 26/600 column

Superdex 30 prep grade (PG) size exclusion chromatography (gel filtration) media prepacked into a HiLoad 26/600 column is optimized for the separation of proteins and peptides, but can also be used with success to separate oligosaccharides.

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Cytiva HiPrep™ Sephacryl™ S-200 HR Columns

Maximum recovery and minimum nonspecific adsorption

Pricing & Availability
Specifications

Particle Size	25 to 75 μm
Height (Metric)	60cm
Content And Storage	4°C to 30°C, 20% Ethanol
pH Range	3 to 11
For Use With (Equipment)	Low to medium pressure AKTA™ systems
Sample Type	Antibodies, serum proteins and mid-size proteins
Quantity	Each
Material	Polypropylene
For Use With (Application)	Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix
Support Type	Allyl Dextran and N, N'-methylenebisacrylamide
Max. Pressure	1.5 bar [0.15 MPa] (22 psi)
Height (English)	23.62 in.

Cytiva HiLoad™ 16/600 Superose™ 6 Prep Grade Preparative SEC Column

A prepacked SEC column for high-resolution purification of large biomolecules and protein complexes with sample volumes up to 5 mL

Pricing & Availability

Cytiva Superose™ Media Prep Grade Lab Packs

High performance gel filtration media for scaleup to preparative purifications of biomolecules

Pricing & Availability
Specifications

Particle Size	30 to 40 μm
Flow Rate	40 cm/hr.
Bed Volume	≈100mL
pH Range	3 to 12
For Use With (Equipment)	AKTA™ design pump or system
Sample Type	Proteins, Peptides, Polynucleotides and wide variety of biomolecules
Quantity	Each
For Use With (Application)	Separations of Proteins and other biomolecules according to size
Support Type	Highly Cross-linked Agarose

Cytiva HiLoad™ Superdex™ 200 PG XK Column

Prepacked XK columns designed for high-resolution preparative gel filtration chromatography.

Pricing & Availability
Specifications

Content And Storage	4 to 30°C, 20% Ethanol

Cytiva Gel Filtration Calibration Kits

Calibrate gel filtration columns simply, reliably. Cytiva Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards.

Pricing & Availability
Specifications

Content And Storage	2° to 8°C
For Use With (Equipment)	High-resolution media and prepacked column
Quantity	Each
For Use With (Application)	Calibration of high-resolution gel filtration media and column such as Superdex™, Superose and Sephacryl