Test
isolated from wheat germ, dissolved in 0.05 M Tris-HCl, 0.15 M NaCl buffer, pH 8.6 and purified by affinity chromatography
The Phytohemagglutinin-L (PHA-L) Solution (500X) is a ready to use solution of PHA-L in aqueous buffer.
Product Type | Phytohemagglutinin-L (PHA-L) Solution |
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Product Line | eBioscience™ |
Wheat germ agglutinin (WGA) binds N-acetylglucosamine, preferentially to dimers and trimers of this sugar. It can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA also interacts with some glycoproteins via sialic acid residues (see succinylated WGA). This conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
NC2606703 RIDASCREEN PEANUT
Peanut agglutinin (PNA) binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and other soluble and membrane-associated glycoproteins and glycolipids. With some exceptions, the PNA receptor sequence is normally sialylated, which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid that is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence. This conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
Dolichos biflorus agglutinin (DBA) binds α-linked N-acetylgalactosamine. Used to establish secretor status in blood group A individuals by hemagglutination inhibition techniques and for blood typing, this lectin has also been used as a general marker of developing renal collecting ducts. This conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
Ulex europaeus agglutinin I (UAE I) binds to glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It is also an established marker for human endothelial cells. This rhodamine-labeled UAE I features a ratio of fluorophore to lectin protein that provides optimal staining (excitation 575 nm, emission 600 nm) and is supplied in solution, essentially free of unconjugated fluorophore.
Spark Violet™ 500 anti-human CD45 [HI30]; Isotype: Mouse IgG1, κ; Reactivity: Human; Apps: FC; Size: 25 tests
CF640R GOAT ANTI-GUINEA PG 1MG
Technical Specifications:Ligand Source:Glycine maxMolecular Weight: 120 kDaCarbohydrate Specificity:GalNAc, GalNAcα1,3GalInhibiting/Eluting Sugar:N-AcetylgalactosamineCarbohydrate for Elution: 0.2MN-AcetylgalactosamineBlood Group Specificity: A>O>BConjugate: FITCForm: LiquidIsoelectric (pI): 5.8-6Storage: -20°CApplication note: Calcium and Manganese are required for binding.
Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V. CF Dye Annexin V Conjugates are provided at 50 ug/mL in buffer with azide as a preservative. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF633 dye has excitation/emission at 630/650 nm.
This product is fluorescently-labeled wheat germ agglutinin (WGA). WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine. WGA conjugates can be used to stain glycoproteins that have these sugar moieties. WGA can be used as a gram stain to fluorescently label gram bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae . CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF488A dye has excitation/emission at 490/515 nm.