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Cytiva Sephacryl™ S-500 High Resolution Media

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

$281.50 - $1014.00


Content And Storage 4° to 30°C
Flow Rate >125 cm/hr.
Particle Size 25 to 75 μm
Bed Volume 19 to 230L
Product Type Sephacryl S-500 HR media
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Chromatography media offer good recovery and low nonspecific adsorption. Enable gel filtration at laboratory and industrial scale, and may be purchased in prepacked columns.

  • For separating polysaccharides, macromolecules with extended structures, and small plasmids
  • Excellent purification over a wide molecular weight range
  • High reproducibility due to long-term chemical and physical stability
  • High flow rates and recoveries
  • Well-suited to industrial-scale use
  • Storage conditions: 20% ethanol; 4° to 30°C
  • Available in a variety of selectivities and volumes

Chemical Stability (stable in all common buffers):

  • 1M acetic acid, 8M urea
  • 6M guanidine-HCl, 1% SDS
  • 2M NaCl, 24% ethanol
  • 30% propanol, 30% acetonitrile
  • 0.5M NaOH (for cleaning)
pH Stability
  • 3 to 11 (long-term and working)
  • 2 to 13 (short-term)
Physical Stability:
  • Negligible volume variation due to changes in pH or ionic strength
  • Autoclavability: At 121°C, pH 7 for 30 minutes (gel only)
  • Antimicrobial agent: 20% ethanol



4° to 30°C
25 to 75 μm
Sephacryl S-500 HR media
40 to 140cm
5.9 in.
Allyl Dextran and N, N'-methylenebisacrylamide
>125 cm/hr.
19 to 230L
Solid Spherical Bead
Allow fast and reproducible purification of proteins, polysaccharides and other macromolecules by gel filtration at laborato ry and industrial scale
3 to 11
Polysaccharides, macromolecules and small plasmids
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