Cytiva Sephacryl™ S-500 High Resolution Media

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

Catalog Number	Mfr. No.	Quantity
45-002-053	Cytiva 17061310	150mL
45-002-054	Cytiva 17061301	750mL

Description

Chromatography media offer good recovery and low nonspecific adsorption. Enable gel filtration at laboratory and industrial scale, and may be purchased in prepacked columns.

• For separating polysaccharides, macromolecules with extended structures, and small plasmids
• Excellent purification over a wide molecular weight range
• High reproducibility due to long-term chemical and physical stability
• High flow rates and recoveries
• Well-suited to industrial-scale use
• Storage conditions: 20% ethanol; 4° to 30°C
• Available in a variety of selectivities and volumes

Chemical Stability (stable in all common buffers):

• 1M acetic acid, 8M urea
• 6M guanidine-HCl, 1% SDS
• 2M NaCl, 24% ethanol
• 30% propanol, 30% acetonitrile
• 0.5M NaOH (for cleaning)

pH Stability

• 3 to 11 (long-term and working)
• 2 to 13 (short-term)

Physical Stability:

• Negligible volume variation due to changes in pH or ionic strength
• Autoclavability: At 121°C, pH 7 for 30 minutes (gel only)
• Antimicrobial agent: 20% ethanol

Specifications

• Content And Storage: 4° to 30°C
• Particle Size: 25 to 75 μm
• Product Type: Sephacryl S-500 HR media
• Diameter (Metric) Inner: 40 to 140cm
• Height (English): 5.9 in.
• Max. Pressure: 100kPa
• Support Type: Allyl Dextran and N, N'-methylenebisacrylamide

• Flow Rate: >125 cm/hr.
• Bed Volume: 19 to 230L
• Format: Solid Spherical Bead
• For Use With (Application): Allow fast and reproducible purification of proteins, polysaccharides and other macromolecules by gel filtration at laborato ry and industrial scale
• Height (Metric): 15cm
• pH Range: 3 to 11
• Sample Type: Polysaccharides, macromolecules and small plasmids