Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids

Catalog Number	Mfr. No.	Bed Volume	Diameter (Metric) Inner	ProductTypeSpecs	Flow Rate
45-002-992	Cytiva 28935606	120 mL	1.6cm		< 1.3 mL/min.
45-002-990	Cytiva 28935604	120 mL	1.6cm		< 0.5 mL/min.
45-002-993	Cytiva 28935607	320 mL	2.6cm		< 0.5 mL/min.

Description

Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).

• Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
• Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
• Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
• Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)

Media    

• Average particle size: 50μm
• Matrix: Allyl dextran and N, N'-methylenebisacrylamide
• pH Stability working range: 3 to 11
• pH Stability cleaning range: 2 to 13
• Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
• Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm

Column

• Material (bed support): Polymethyl-Methacrylate

HiPrep 16/60 Sephacryl S-400 HR

• Media:
• Fractionation [Mr] globular proteins: 2 x 104^-8 x 106
• Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
• Fractionation range [Mr] DNA exclusion limit:    271bp
• Fractionation [Mr] Dextrans: 1 x 104^-2 x 106

HiPrep 16/60 Sephacryl S-500 HR

• Media:
• Exclusion limit for Dextrans: 100 x 106
• Fractionation range [Mr] DNA exclusion limit: 1078bp
• Fractionation [Mr] Dextrans: 4 x 104^-2 x 107

Specifications

• Quantity: Each
• For Use With (Equipment): Low to medium pressure AKTA™ systems or pumped chromatography systems
• For Use With (Application): Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
• Height (Metric): 60cm
• Max. Pressure: 1.5 bar [0.15 MPa] (22 psi)
• Support Type: Allyl Dextran and N,N'-methylenebisacrylamide

• Particle Size: 25 to 75 μm
• Product Type: Sephacryl HR Pre-packed Gel Filtration Column
• Height (English): 23.62 in.
• Material: Polypropylene
• pH Range: 3 to 11
• Sample Type: Polysaccharides, macromolecules with extended structures and even small plasmids