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Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids

$1095.00 - $1470.00

Specifications

Quantity Each
Particle Size 25 to 75 μm
For Use With (Equipment) Low to medium pressure AKTA™ systems or pumped chromatography systems
For Use With (Application) Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
Height (English) 23.62 in.
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Products 3
Catalog Number Mfr. No. Bed Volume Diameter (Metric) Inner Product Type Flow Rate Price Quantity & Availability  
Catalog Number Mfr. No. Bed Volume Diameter (Metric) Inner Product Type Flow Rate Price Quantity & Availability  
45-002-992
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Cytiva
28935606
120 mL 1.6cm HiPrep 16/60 Sephacryl S-500 HR, Sephacryl HR Pre-packed Gel Filtration Column < 1.3 mL/min.
Each for $1,095.00
Add to Cart
 
45-002-990
View Documents
Cytiva
28935604
120 mL 1.6cm HiPrep 16/60 Sephacryl S-400 HR, Sephacryl HR Pre-packed Gel Filtration Column < 0.5 mL/min.
Each for $1,095.00
Add to Cart
 
45-002-993
View Documents
Cytiva
28935607
320 mL 2.6cm HiPrep 26/60 Sephacryl S-500 HR, Sephacryl HR Pre-packed Gel Filtration Column < 0.5 mL/min.
Each for $1,470.00
Add to Cart
 
Description

Description

Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).

  • Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
  • Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
  • Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
  • Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)

Media    

  • Average particle size: 50μm
  • Matrix: Allyl dextran and N, N'-methylenebisacrylamide
  • pH Stability working range: 3 to 11
  • pH Stability cleaning range: 2 to 13
  • Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
  • Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm
Column
  • Material (bed support): Polymethyl-Methacrylate

HiPrep 16/60 Sephacryl S-400 HR

  • Media:
  • Fractionation [Mr] globular proteins: 2 x 104-8 x 106
  • Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
  • Fractionation range [Mr] DNA exclusion limit:    271bp
  • Fractionation [Mr] Dextrans: 1 x 104-2 x 106
HiPrep 16/60 Sephacryl S-500 HR
  • Media:
  • Exclusion limit for Dextrans: 100 x 106
  • Fractionation range [Mr] DNA exclusion limit: 1078bp
  • Fractionation [Mr] Dextrans: 4 x 104-2 x 107

Specifications

Specifications

Each
Low to medium pressure AKTA™ systems or pumped chromatography systems
23.62 in.
Polypropylene
3 to 11
Polysaccharides, macromolecules with extended structures and even small plasmids
25 to 75 μm
Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
60cm
1.5 bar [0.15 MPa] (22 psi)
Allyl Dextran and N,N'-methylenebisacrylamide
HiPrep 16/60 Sephacryl S-500 HR, Sephacryl HR Pre-packed Gel Filtration Column
SDS
Documents

Documents

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