Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids

Catalog Number	Mfr. No.	Bed Volume	Diameter (Metric) Inner	Product Type	Flow Rate
45-002-992	Cytiva 28935606	120 mL	1.6cm	HiPrep 16/60 Sephacryl S-500 HR	< 1.3 mL/min.
45-002-990	Cytiva 28935604	120 mL	1.6cm	HiPrep 16/60 Sephacryl S-400 HR	< 0.5 mL/min.
45-002-993	Cytiva 28935607	320 mL	2.6cm	HiPrep 26/60 Sephacryl S-500 HR	< 0.5 mL/min.

Description

Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).

• Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
• Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
• Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
• Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)

Media    

• Average particle size: 50μm
• Matrix: Allyl dextran and N, N'-methylenebisacrylamide
• pH Stability working range: 3 to 11
• pH Stability cleaning range: 2 to 13
• Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
• Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm

Column

• Material (bed support): Polymethyl-Methacrylate

HiPrep 16/60 Sephacryl S-400 HR

• Media:
• Fractionation [Mr] globular proteins: 2 x 104^-8 x 106
• Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
• Fractionation range [Mr] DNA exclusion limit:    271bp
• Fractionation [Mr] Dextrans: 1 x 104^-2 x 106

HiPrep 16/60 Sephacryl S-500 HR

• Media:
• Exclusion limit for Dextrans: 100 x 106
• Fractionation range [Mr] DNA exclusion limit: 1078bp
• Fractionation [Mr] Dextrans: 4 x 104^-2 x 107

Specifications

• Quantity: Each
• For Use With (Equipment): Low to medium pressure AKTA™ systems or pumped chromatography systems
• Height (English): 23.62 in.
• Material: Polypropylene
• pH Range: 3 to 11
• Sample Type: Polysaccharides, macromolecules with extended structures and even small plasmids

• Particle Size: 25 to 75 μm
• For Use With (Application): Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
• Height (Metric): 60cm
• Max. Pressure: 1.5 bar [0.15 MPa] (22 psi)
• Support Type: Allyl Dextran and N,N'-methylenebisacrylamide