Antibody Fragmentation Kits
Antibody Fragmentation Kits
MilliporeSigma™ Calbiochem™ IgG2a from Mouse Myeloma
Derived from mouse myeloma.
Thermo Scientific™ Pierce™ Fab Micro Preparation Kit
Digest human or mouse IgG antibodies with immobilized papain protease and purify Fab fragments using Protein A agarose.
Thermo Scientific™ Pierce™ Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit
Efficiently digest mouse IgG1 antibodies into Fab or F(ab')2 fragments with immobilized ficin protease.
Thermo Scientific™ Pierce™ F(ab')2 Preparation Kit
Selectively digest whole human and other IgG antibodies with immobilized pepsin protease to make F(ab')2 fragments that retain antigen binding activity.
Thermo Scientific™ Pierce™ Mouse IgG1 Fab and F(ab')2 Preparation Kit
Efficiently digest mouse IgG1 antibodies into Fab or F(ab')2 fragments with immobilized ficin protease.
Thermo Scientific™ Pierce™ Fab Preparation Kit
Digest human or mouse IgG antibodies with immobilized papain protease and purify Fab fragments using Protein A agarose.
Thermo Scientific™ Pierce™ F(ab')2 Micro Preparation Kit
Selectively digest whole human and other IgG antibodies with immobilized pepsin protease to make F(ab')2 fragments that retain antigen binding activity.
GENOVIS INC FRAGIT KIT CLEAVE AND PURIFY
5 FragIT MicroSpin columns + 5 Fc fragment affinity columns. FragIT kit is an extremely rapid and easy way of creating and isolating F(ab')2 and Fc fragments from IgG. The kit consist of two spin columns - one for fragmentation (FragIT column) and one for purification of F(ab')2 and isolation of Fc fragments. The FragIT column is FabRICATOR enzyme immobilized on agarose and the second column has affinity ligands selective for Fc fragments.
Beckman Coulter VersaComp Antibody Capture Kit, 2 Vials, LUO
VersaComp Antibody Capture Bead kit provides both positive and negative microspheres that can be used to set compensation for multicolor flow cytometry experiments. The positive population captures conjugates in single color stains and recognizes all mouse and rat isotypes, most hamster isotypes and rabbit polyclonal IgG. The negative population provides fluorescence similar to unstained cells across the different excitation/emission wavelength combinations. This combination can be used to set up compensation to correct for spectral overlap.
GENOVIS INC FRAGIT KIT CLEAVE/PURIFY 10MG
FragIT™ Kit MicroSpin. 10 ml1 settled resin of FabRICATOR® (IdeS) immobilized on highly cross-linked agarose. Storage temp 4-8°C. White resin. Sterility - Absence of bacterial growth3. Stability - 1 year at 4-8°C
Proteochem Inc Immobilized Pepsin 5 ml
Immobilized Pepsin resin (Pepsin Agarose) is commonly used to create F(ab’)2 fragments from antibodies. By immobilizing pepsin autolysis and protease contamination is essentially eliminated. Immobilized Pepsin gel hydrolyzes protein non-specifically at pH 1.0 to 3.0, with an optimum activity at pH 1.0. ProteoChem's highly active pepsin is covalently bound to crosslinked 6% beaded agarose resin and its specific activity is > 6,100 units/ml resin (one unit is defined as releasing 0.001 A280 as TCA hydrolyzed/min. at 37°C). ProteoChem's Immobilized Pepsin is packaged as a slurry in buffer at pH 4.5 containing 50% glycerol, 100 mM Sodium Acetate, 0.05% NaN3.
