Test
Digest human or mouse IgG antibodies with immobilized papain protease and purify Fab fragments using Protein A agarose.
Selectively digest whole human and other IgG antibodies with immobilized pepsin protease to make F(ab')2 fragments that retain antigen binding activity.
Digest human or mouse IgG antibodies with immobilized papain protease and purify Fab fragments using Protein A agarose.
Efficiently digest mouse IgG1 antibodies into Fab or F(ab')2 fragments with immobilized ficin protease.
Efficiently digest mouse IgG1 antibodies into Fab or F(ab')2 fragments with immobilized ficin protease.
Selectively digest whole human and other IgG antibodies with immobilized pepsin protease to make F(ab')2 fragments that retain antigen binding activity.
FragIT™ Kit MicroSpin. 10 ml1 settled resin of FabRICATOR® (IdeS) immobilized on highly cross-linked agarose. Storage temp 4-8°C. White resin. Sterility - Absence of bacterial growth3. Stability - 1 year at 4-8°C
FragIT Z kit, Digest and Purify up to 5 x 0.5 mg FragIT Z kit, Digest and Purify up to 5 x 0.5 mgFragIT Z kit for creation and isolation of F(ab’)2 and Fc fragments from 5 x 0.5 mg mouse IgG2a. The kit consist of two spin columns – one for fragmentation (FragIT Z column) and one for purification of F(ab’)2 and isolation of Fc fragments.The FragIT Z column is FabRICATOR Z enzyme immobilized on agarose and the second column has affinity ligands selective for Fc fragments.
NC1664638 FRAGIT KIT DIGEST/PUR 10X0.5MG
Immobilized Pepsin resin (Pepsin Agarose) is commonly used to create F(ab’)2 fragments from antibodies. By immobilizing pepsin autolysis and protease contamination is essentially eliminated. Immobilized Pepsin gel hydrolyzes protein non-specifically at pH 1.0 to 3.0, with an optimum activity at pH 1.0. ProteoChem's highly active pepsin is covalently bound to crosslinked 6% beaded agarose resin and its specific activity is > 6,100 units/ml resin (one unit is defined as releasing 0.001 A280 as TCA hydrolyzed/min. at 37°C). ProteoChem's Immobilized Pepsin is packaged as a slurry in buffer at pH 4.5 containing 50% glycerol, 100 mM Sodium Acetate, 0.05% NaN3.
VersaComp Antibody Capture Bead kit provides both positive and negative microspheres that can be used to set compensation for multicolor flow cytometry experiments. The positive population captures conjugates in single color stains and recognizes all mouse and rat isotypes, most hamster isotypes and rabbit polyclonal IgG. The negative population provides fluorescence similar to unstained cells across the different excitation/emission wavelength combinations. This combination can be used to set up compensation to correct for spectral overlap.
NC0919531 FRAGIT MICROSPIN CLEAVES 2 X
NC1458861 FRAGIT KIT MICROSPIN 0.5MG
Reagents for capture of human antibody Fab fragments for in biomolecular interaction analyses.