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Thermo Scientific™ Pierce™ Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit

Efficiently digest mouse IgG1 antibodies into Fab or F(ab')2 fragments with immobilized ficin protease.

Manufacturer:  Thermo Scientific™ 44680

Catalog No. PI44680


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Description

Description

Thermo Scientific™ Pierce Mouse IgG&subscript_1; Fab and F(ab')&subscript_2; Preparation Kits use immobilized ficin protease to efficiently digest mouse IgG&subscript_1; into Fab or F(ab')&subscript_2; fragments, depending on the chosen reaction conditions.

The kits use ficin protease to enzymatically digest the Fc portion of whole IgG to yield Fab or F(ab')2 fragments. Because the ficin protease is supplied in immobilized form as beaded agarose resin, the digestion reaction is easily stopped by removing the resin from the IgG solution; the result is digest products that are enzyme-free. An optimized procedure and high-performance desalting and Protein A spin columns make these kits easy to use.

Highlights:

  • Enzyme-free digestion products – immobilized ficin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the protease

  • Optimized for mouse IgG1 – the kit procedure is specifically optimized for monoclonal mouse IgG1 antibodies, providing the best possible yield for this antibody subclass

  • High capacity – kit provides for digestion and purification from as much as 4mg of IgG at a time. Use the standard size kit for fragmenting 0.25 to 4mg IgG, and the micro kit for 25 to 250μg IgG

  • Complete and easy to use – kits include all reagents, spin columns and accessories needed to prepare and purify antibody fragments

Includes:

Ficin agarose resin, cysteine, digestion buffer, Protein A columns with binding and elution buffers, spin desalting columns and accessories

Specifications

Specifications

Pierce™
0.8 mL Resin Kit
Safety and Handling

Safety and Handling

SDS
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Product Certifications
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