Nucleic Acid Labeling and Detection
Nucleic Acid Labeling and Detection
Nucleic acid labeling and detection products are used to add tags or labels to nucleic acids to enable detection or purification. The tagged nucleic acids are referred to as 'probes.'
Nucleotide labeling may include radioactive phosphates, biotin, fluorophores, and various enzymes. Bioconjugation methods that produce nucleic acid probes can also be adapted to attach them to other molecules (for targeted delivery) and surfaces (to facilitate immobilization).
Although nucleic acid probes can be labeled during synthesis, custom oligonucleotide probes can be expensive so many researchers choose to label their own.
Quick and efficient benchtop oligonucleotide labeling can be performed for making smaller probe amounts or when multiple probes with the same label are needed. Enzymatic methods are affordable for small-scale probe generation; chemical methods are more suited to larger scale reactions.
Probes can be labeled at either end of the oligonucleotide or randomly incorporated throughout the sequence. The choice of method depends on the degree of labeling required and whether changes will prevent theexpected interactions. Nucleic acid hybridization reactions can benefit from random label incorporation; protein interactions may require end-labelling.
Summary of Methods
Enzymatic
- TdT: ssDNA
- T4 RNA Ligase: ssDNA, RNA
- T4 PNK: ssDNA, RNA
- DNA Polymerase: DNA, RNA
- RNA Polymerase: RNA
Chemical
- Periodate: RNA
- EDC: DNA, RNA
- Nonspecific crosslinkers: DNA, RNA
Detection methods are biotin-based, chemiluminescent, fluorescent, radioactive, or the combination of several techniques. Tests may include nucleic acid tests for pathogen detection and fluorescent in situ hybridization (FISH) for specific DNA sequences in chromosomes.
Thermo Scientific™ Pierce™ RNA 3' End Biotinylation Kit
Biotinylate 3-prime terminal ends of RNA probes for non-radioactive EMSA and other nucleic acid detection methods.
Cytiva Amersham™ Streptavidin-Biotinylated HRP Complex
Streptavidin conjugated to reporter enzyme molecules for use in blotting and in situ hybridization applications
Cytiva Amersham™ Megaprime™ DNA Labeling System
For labeling DNA probes to specific activities >1 × 109dpm/μg in 10 minutes
Cytiva Amersham™ ECL™ Direct Nucleic Acid Labeling/Detection System
Use for fast and easy detection of medium-to-high-target amounts. Cytiva Life Sciences™ Amersham™ ECL™ Direct Nucleic Acid Labeling/Detection System are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction
Cytiva Amersham™ Rediprime II DNA Labeling System
Long shelf-life and simple, fast protocol. Amersham™ Rediprime II DNA Labeling System consists of premixed, individually dispensed, random-prime reactions, freeze-dried for ambient stability.
Cytiva Amersham™ Streptavidin-HRP Conjugate
Streptavidin conjugated to reporter enzyme molecules for use in blotting and in situ hybridization applications
Thermo Scientific™ DecaLabel DNA Labeling Kit
Thermo Scientific™ Fermentas DecaLabel DNA Labeling Kit is a complete system for fast synthesis of radiolabeled DNA probes of high specific activity.
Thermo Scientific™ Pierce™ Biotin 3' End DNA Labeling Kit
Biotinylate single-stranded DNA primers for non-radioactive EMSA and other nucleic acid detection methods.
Thermo Scientific™ LightShift™ Poly (dI-dC)
Identify and characterize protein-DNA binding interactions by EMSA with this extraordinarily robust and sensitive system.
Thermo Scientific™ LightShift™ EMSA Optimization and Control Kit
Identify and characterize protein-DNA binding interactions by EMSA with this extraordinarily robust and sensitive system.
Thermo Scientific™ Pierce™ RNA 3' End Desthiobiotinylation Kit
Perform easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins.
Thermo Scientific™ LightShift™ Chemiluminescent EMSA Kit
Identify and characterize protein-DNA binding interactions by EMSA with this extraordinarily robust and sensitive system.
MilliporeSigma™ Novagen™ EMSA Accessory Kit
Used for testing DNA binding proteins.
MilliporeSigma™ Calbiochem™ Dextran Sulfate, Sodium Salt, Molecular Biology Grade
Used as anticoagulant.
Thermo Scientific™ tRNA for LightShift™ Chemiluminescent RNA EMSA Kit
Study RNA-protein interactions with this non-radioactive EMSA kit for gel shift assays.
Thermo Scientific™ LightShift™ Chemiluminescent RNA EMSA Kit
Study RNA-protein interactions with this non-radioactive EMSA kit for gel shift assays.
Cytiva AlkPhos Direct™ Hybridization Buffer for Direct Labeling System
Fast and convenient system for all high-sensitivity applications using DNA, RNA, or oligonucleotide probes
Cytiva Amersham™ ECF™ Substrate for Western Blotting
Used for detection of protein blots with fluorescence scanning equipment. Cytiva Life Sciences™ Amersham™ ECF™ Substrate for Western Blotting uses a fluorescent substrate for alkaline phosphatase-based detection of protein blots.
Cytiva Amersham™ AlkPhos Direct™ Labeling Modules
Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes. AlkPhos Direct Labeling Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase.
Cytiva Amersham™ ECL™ Blocking Agent
Use for blocking nitrocellulose and PVDF membranes. Cytiva Life Sciences™ Amersham™ ECL™ Blocking Agent blocks membranes to be used with ECL and ECL Plus™ Western blotting detection reagents.
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Thermo Scientific™ Chemiluminescent Nucleic Acid Detection Module Kit
Detect biotin-labeled nucleic acid probes in various membrane blotting applications with this complete chemiluminescent detection system.
Mirus Bio™ Label IT™ Nucleic Acid Labeling Kit, Biotin
Efficiently and reproducibly attach marker molecules to DNA or RNA in a simple one-step reaction within minutes. Covalently attach labels without impacting hybridization performance.
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Mirus Bio™ Label IT Nucleic Acid Digoxin
Label IT Nucleic Acid Labeling Reagents for efficient, direct, non-enzymatic labeling of DNA and RNA.
Mirus Bio™ Label IT™ Amine Modifying Reagent
Attach amine functional groups covalently to nucleic acids. The simplicity of this chemical labeling reaction allows one to control the level of modification. For labeling with hydrazine, amine, or amine-reactive dyes and attachment to activated glass surfaces or hydrazide-coated beads or plates.
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Mirus Bio™ Label IT Nucleic Acid DNP
Label IT Nucleic Acid Labeling Reagents for efficient, direct, non-enzymatic labeling of DNA and RNA.
Mirus Bio™ Label IT Nucleic Acid TM-Rho
Label IT Nucleic Acid Labeling Reagents for efficient, direct, non-enzymatic labeling of DNA and RNA.
Mirus Bio™ Label IT Plasmid Cy3
Label IT Plasmid Delivery Control is fluorescently labeled plasmid ideal for in vitro and in vivo tracking.
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Mirus Bio™ Label IT™ Nucleic Acid Labeling Kit, Fluorescein
Efficiently and reproducibly attach marker molecules to DNA or RNA in a simple one-step reaction within minutes. Covalently attach labels without impacting hybridization performance.
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Mirus Bio™ Label IT™ Nucleic Acid Labeling Kit, CX-Rhodamine
Efficiently and reproducibly attach marker molecules to DNA or RNA in a simple one-step reaction within minutes. Covalently attach labels without impacting hybridization performance.
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Mirus Bio™ Label IT™ Nucleic Acid Labeling Kit, Cy5
Efficiently and reproducibly attach marker molecules to DNA or RNA in a simple one-step reaction within minutes. Covalently attach labels without impacting hybridization performance.
Promotions are available