Invitrogen™ Qubit™ dsDNA Quantification Assay Kits

For highly selective detection of dsDNA over RNA, even in the presence of contaminants. The Qubit dsDNA Assay Kits are accurate from 10 pg/uL to 100 ng/uL, and 100 pg/uL to 1,000 ng/uL, respectively.

Catalog Number	Mfr. No.	Assay	Quantitation Range	No. of Reactions
Q32850	Invitrogen™ Q32850	dsDNA Quantitation, Broad Range	4 to 2000 ng	100 Reactions
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Q32853	Invitrogen™ Q32853	dsDNA Quantitation, Broad Range	4 to 2000 ng	500 Reactions
Q32851	Invitrogen™ Q32851	dsDNA Quantitation, High Sensitivity	0.1 to 120 ng	100 Reactions
Q32854	Invitrogen™ Q32854	dsDNA Quantitation, High Sensitivity	0.1 to 120 ng	500 Reactions

Description

Achieve accurate and precise quantification of dsDNA with Qubit dsDNA Assay Kits. These dsDNA quantification kits enable quick and selective detection of low and high abundance DNA samples, and can distinguish dsDNA from ssDNA, RNA, protein, and free nucleotides. Contaminants such as salts, solvents, or detergents are well-tolerated.

Qubit dsDNA HS and BR Assay Kits, designed for use with Qubit Fluorometers, are highly selective for double-stranded DNA (dsDNA) over single-stranded DNA (ssDNA), RNA, protein, and free nucleotides. All kits provide concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 μL and 20 μL is acceptable), and read the concentration using a Qubit Fluorometer.

Qubit dsDNA HS Assay Kit
The Qubit dsDNA HS (High Sensitivity) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of sensitive DNA samples. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.005 to 120 ng/μL, providing a detection range of 0.1−120 ng.

Qubit dsDNA BR Assay Kit
The Qubit dsDNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of DNA samples. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.2 to 2,000 ng/μL, providing a detection range of 4−2,000 ng.

Notes
• Qubit dsDNA HS and BR Assay kits can be used with any Qubit Fluorometer
• Use with thin-wall, clear, 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 Fluorometer and 8 x 200-μL tube strips (Cat. No. Q33252) for the Qubit Flex Fluorometer

Specifications

• Detection Method: Fluorescence
• For Use With (Equipment): Qubit Fluorometer
• Shipping Condition: Room Temperature

• Excitation/Emission: 510/527
• Product Line: Qubit