Invitrogen™ Quant-iT™ dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)

For highly selective quantitation of double-stranded DNA over RNA, the Quant-iT dsDNA Assay Kits, high sensitivity and broad range, produce fluorescence signals that are linear in the ranges of 0.2-100 ng and 2-1000 ng of DNA, respectively.

Catalog Number	Mfr. No.	Assay	Quantitation Range
Q33120	Invitrogen™ Q33120	dsDNA Quantitation, high sensitivity	0.2 to 100 ng
Q33130	Invitrogen™ Q33130	dsDNA Quantitation, broad range	4 to 1000 ng

Description

Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and the Quant-iT Broad-Range dsDNA Assay Kit provide concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. These DNA assay kits are highly selective for double-stranded DNA over RNA, and, in the ranges of 0.2–100 ng DNA (for the HS dsDNA Assay Kit) and 4–1000 ng DNA (for the BR dsDNA Assay Kit), the fluorescence signal is linear.

The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Simply dilute the reagent 1:200, load 200 μL into the wells of a microplate, add 1–20 μL sample, mix, then read the fluorescence.

The assays are highly selective for double-stranded DNA over RNA, and, in the range of 0.2–100 ng for the HS assay or 4–1000 ng for the BR assay, the fluorescence signal is linear with DNA. The assays are performed at room temperature, and the signal is stable for three hours. Common contaminants such as salts, solvents, detergents, and protein are well tolerated in these assays.

Specifications

• Detection Method: Fluorescence
• No. of Reactions: 1000 Assays (200 μL assay volume)
• Shipping Condition: Room Temperature

• For Use With (Equipment): Microplate Reader
• Product Line: Quant-iT