Test
Designed for use in compensation with all fluorochromes excited by ultraviolet, violet, blue, green, yellow-green, and red lasers. UltraComp eBeads™ react with antibodies of mouse, rat, and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test.
For Use With (Equipment) | Flow Cytometer |
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Product Type | Compensation Beads |
Product Line | eBeads™ |
Spherical particles that can be stained with individual fluorochrome-conjugated antibodies for use as single-color compensation controls. OneComp eBeads react with antibodies of mouse, rat and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test
For Use With (Equipment) | Flow Cytometer |
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Product Type | Compensation Beads |
Product Line | eBeads™ |
Includes | Water insoluble fluorescent dyes |
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Content And Storage | 4°C |
Format | Liquid |
Product Type | Fluorescent Particles |
Color | Clear |
Volume (Metric) | 2 mL |
Material | Polystyrene |
Concentration | 1% |
Certifications/Compliance | RUO |
For Use With (Application) | Flow Cytometry Calibration and Alignment |
A buffered saline solution containing fetal bovine serum and sodium azide (0.09%) as a preservative. Designed for use in immunofluorescent staining protocols of cells in suspension.
Product Type | Staining Buffer |
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Product Line | eBioscience™ |
Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.
Provide greater durability and chemical stability when using solvents with these monodisperse silica microspheres used in instrument calibration and as spacers.
Product Type | Silica Particle Size Standard |
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Calibrated suspension of ∼4 μm beads that encapsulate dyes that are excitable across a wide range of UV-IR wavelengths and brightly fluoresce in the emission range of 385-860 nm.
Fluorescent marker for macrostructures, e.g. microtubules; Aldehyde modification for coupling with amines
Content And Storage | 2°C to 8°C |
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Concentration | 2% solids |
Invitrogen™ eBioscience™ Essential Human Treg Phenotyping Kit is designed for flow cytometry-based identity testing of regulatory T-cells (Tregs).
Cocktail of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines.
Product Type | Cell Stimulation Cocktail |
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Product Line | eBioscience™ |
For staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines. Should be used with the Permeabilization Buffer. All three components sold together as the Foxp3 / Transcription Factor Staining Buffer Set.
Designed for use in preparation of living cells for intracellular staining and flow cytometric analysis. A protein transport inhibitor (Brefeldin A) is included for treatment of cells during culture, to cause accumulation of cytokine protein at the Golgi and enhance resulting staining.