Flow Cytometry Compensation, Counting and Calibration
Flow Cytometry Compensation, Counting and Calibration
Thermo Scientific™ 8000 Series Silica Particle Size Standards
Provide greater durability and chemical stability when using solvents with these monodisperse silica microspheres used in instrument calibration and as spacers.
BD Sphero™ Rainbow Calibration Particles
For flow cytometry calibration and alignment
Micro Particles Based on Melamine Resin, Carboxylate-Modified, FITC-marked, Analytical Standard, Size: 1 m, MilliporeSigma™ Supelco™
Fluorescent marker for macrostructures, e.g. microtubules; Aldehyde modification for coupling with amines
BD Sphero™ Fluorescent Particles
For flow cytometry calibration and alignment
BD FACSArray™ Daily QC Particles
Consist of three discrete fluorescent or non-fluorescent particles for daily instrument quality control to confirm consistent instrument performance
Novus Biologicals™ Blank Comp-Bead Particles
The Blank Compensation Beads serve as a negative control for setting compensation in multicolor flow cytometry.
Novus Biologicals™ GFP Comp-Bead Particles
The GFP Compensation Beads serve as an easy-to-use single color compensation control in multicolor flow cytometry. The surface of the particle is labeled with GFP.
Novus Biologicals™ Absolute Rainbow Cell Count Particle Set
The Absolute Rainbow Cell Count Particles are designed as reference particles with a known number of particles per mL for counting the absolute cell number in flow cytometry.
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Novus Biologicals™ Max Rainbow Fluorescent Particle Set (0.1 - 0.3 um)
Used for performance tracking in multiple channels (UV, violet, green, yellow, orange, far red, and IR)
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Novus Biologicals™ Rainbow Calibration Particle Set (8 peaks)
Used for long term performance tracking or routine calibration of flow cytometers
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Novus Biologicals™ Rainbow Calibration Particle Set (6 peaks)
Used for long term performance tracking or routine calibration of flow cytometers
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Novus Biologicals™ Rainbow Calibration Particle Set (8 peaks - EAL01)
Used for long term performance tracking or routine calibration of flow cytometers
Invitrogen™ eBioscience™ Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A)
Designed for use in preparation of living cells for intracellular staining and flow cytometric analysis. A protein transport inhibitor (Brefeldin A) is included for treatment of cells during culture, to cause accumulation of cytokine protein at the Golgi and enhance resulting staining.
Invitrogen™ 123count eBeads™ Counting Beads
Intended for use in absolute counting of cells or other particles by flow cytometry. 123count eBeads are 7μm microparticles containing encapsulated dyes compatible with blue (488nm) and violet (405nm) excitation sources and emitting fluorescence between approximately 500nm and 750nm.
Invitrogen™ UltraComp eBeads™ Compensation Beads
Designed for use in compensation with all fluorochromes excited by ultraviolet, violet, blue, green, yellow-green, and red lasers. UltraComp eBeads™ react with antibodies of mouse, rat, and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test.
Invitrogen™ OneComp eBeads™ Compensation Beads
Spherical particles that can be stained with individual fluorochrome-conjugated antibodies for use as single-color compensation controls. OneComp eBeads react with antibodies of mouse, rat and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test
Invitrogen™ eBioscience™ Fixation/Permeabilization Diluent
Intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.
Invitrogen™ eBioscience™ Permeabilization Buffer (10X)
Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.
Invitrogen™ eBioscience™ Flow Cytometry Staining Buffer
A buffered saline solution containing fetal bovine serum and sodium azide (0.09%) as a preservative. Designed for use in immunofluorescent staining protocols of cells in suspension.
Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set
Formulated and optimized for staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines
Invitrogen™ eBioscience™ Cell Stimulation Cocktail (500X)
Cocktail of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines.
Invitrogen™ eBioscience™ Human Regulatory T Cell Staining Kit #3
Contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells.
Invitrogen™ eBioscience™ Mouse Regulatory T Cell Staining Kit #3
Formulated & optimized for the staining of mouse splenocytes with the FJK-16s monoclonal antibody. eBioscience™ Mouse Regulatory T Cell Staining Kit #3 includes: Anti-Mouse CD4 FITC, CD25 PE & CD16/32, Anti-Mouse/Rat Foxp3 PE-Cy5, Rat IgG2a Isotype Control PE-Cy5, stain buffer, fixation concentrate.
Invitrogen™ eBioscience™ Human Th17 Cytokine Staining Panel
Includes all reagents needed for simultaneous flow cytometric detection of all the major cytokines produced by the Th17 lineage (IL-17A, IL-17F, IL-21 and IL-22).
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