Antibody Panels and Kits

Combinations of antibody-related products that may include sets of antibodies in pairs and cocktails, control particles, buffers, and other kits and panels; for use in specific applications such as immunoassays and T-cell activation.

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MilliporeSigma™ Upstate™ Dimethyl-Histone H3 (Lys27)α, Rabbit Monoclonal, ChIP Validated Antibody and Primer Set

Rabbit Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C for one year from date of shipment
Host Species	Rabbit
Applications	ChIP Assay,Western Blot
Form	Culture Supernatant
Gene Accession No.	Q71DI3
Isotype	IgG
Includes	This ChIPAb+ Dimethyl-Histone H3 (Lys27) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	Dimethyl-Histone H3 (Lys27)α
Regulatory Status	RUO
Gene Symbols	H3, H3.2, H3/M, H3/m, H3/o, H3F2, H3FM, MGC9629, OTTHUMP00000014041
Purification Method	Unpurified supernatant
Gene ID (Entrez)	NP_003484.1
Formulation	Anti-Dimethyl-Histone H3 (Lys27) (rabbit monoclonal). One vial containing 50μL of cultured supernantant in 0.05% sodium azide. Negative Control Supernatant. One vial containing 100μL of rabbit cultured supernatant in 0.05% sodium azide. ChIP Primers, human β-globin. One vial containing 75μL of 5μM of each primer specific for the human β-globin promoter. FOR: AGG ACA GGT ACG GCT GTC ATC; REV: TTT ATG CCC AGC CCT GGC TC
Immunogen	Peptide containing the sequence (ARme2KSA) in which me2 corresponds to dimethyl lysine at residue 27 of human histone H3.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	This antibody recognizes Histone H3 dimethylated on Lys27.

MilliporeSigma™ Upstate™ ChIPAb+™ HDAC1 Rabbit Monoclonal, ChIP Validated Antibody and Primer Set

Rabbit Monoclonal Antibody

Pricing & Availability
Specifications

Includes	This ChIPAb+ HDAC1 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	ChIPAb+™ HDAC1
Regulatory Status	RUO
Content And Storage	-20°C for one year from date of shipment; Aliquot to avoid repeated freezing and thawing; For maximum recovery of product centrifuge the original vial after thawing and prior to removing the cap
Host Species	Rabbit
Applications	ChIP Assay,Immunocytochemistry,Western Blot
Form	Purified
Gene ID (Entrez)	NP_032254
Formulation	Anti-HDAC1 (rabbit polyclonal). One vial containing 50μg of Anti-HDAC1 IgG in PBS with 1mg/mL BSA and ≤0.1% sodium azide before the addition of glycerol to 30%. Concentration: 0.7mg/mL Normal Rabbit IgG. One vial containing 125μg of rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide. ChIP Primers p21. One vial containing 75μL of 5μM of each primer specific for a region of the human p21 (WAF1/CIP1/CDKN1A) promoter. FOR: CCC ACA GCA GAG GAG AAA GAA; REV: CTG GAA ATC TCT GCC CAG ACA
Immunogen	Synthetic peptide corresponding to amino acids 467-482 of human HDAC1.
Classification	Monoclonal
Primary or Secondary	Primary

MilliporeSigma™ Upstate™ Dimethyl-Histone H4 (Arg3) Symmetric, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Rabbit
Applications	ChIP Assay,Dot Blot,Western Blot
Gene Accession No.	P62805
Includes	This ChIPAb+ Dimethyl-Histone H4 (Arg3) Symmetric -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	Dimethyl-Histone H4 (Arg3) Symmetric
Regulatory Status	RUO
Gene Symbols	HIST1H4A, H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, H4FB, H4/K, HIST1H4I, H4/N, HIST1H4B, H4FE, H4/M, H4/a, HIST1H4E, HIST4H4, HIST2H4A, H4FC, H4FI, H4/H, HIST1H4C, H4/G, HIST1H4K, H4FJ, H4FD, H4/I, H4/B, H4/D, H4/C, HIST1H4J, HIST1H4D, H4/E
Purification Method	Affinity Purified
Gene ID (Entrez)	NP_001029249
Formulation	Anti-Dimethyl-Histone H4 (Arg3) Symmetric (rabbit polyclonal). One vial containing 17.5μg of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide before the addition of glycerol to 30%. Concentration: 0.7μg/μL. Normal Rabbit IgG. One vial containing 125μg of Rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide. ChIP Primers, human β-globin. One vial containing 75μL of 5μM of each primer specific for the human β-globin promoter. FOR: AGG ACA GGT ACG GCT GTC ATC; REV: TTT ATG CCC AGC CCT GGC TC
Immunogen	KLH-conjugated linear peptide corresponding to human Histone H4 when symmetrically dimethylated at Arg3.
Classification	Polyclonal
Primary or Secondary	Primary
Test Specificity	This antibody recognizes Histone H4 symmetrically dimethylated at Arg3.

MilliporeSigma™ Upstate™ Histone H3 (CT), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Rabbit
Applications	ChIP Assay,Dot Blot,Immunocytochemistry,Inhibition Assays,Western Blot
Form	Serum
Gene Accession No.	Q16695
Includes	This ChIPAb+ Histone H3 (C-Term) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	Histone H3 (CT)
Regulatory Status	RUO
Gene Symbols	HIST3H3, H3FT, MGC126886, H3t, MGC126888, H3T, H3/g, H3.4, H3/t
Purification Method	Unpurified
Gene ID (Entrez)	NM_002107.3
Formulation	Anti-Histone H3 (C-Term) (rabbit polyclonal). One vial containing 25μL of rabbit antiserum diluted 1:2 in storage buffer (0.02M Phosphate, 0.25M NaCl, 0.1% sodium azide) before the addition of glycerol to 30%. Normal Rabbit Serum. One vial containing 25μL of antiserum containing 0.05% sodium azide. ChIP Primers, human β-globin. One vial containing 75μL of 5μM of each primer specific for the human β-globin promoter. FOR: AGG ACA GGT ACG GCT GTC ATC; REV: TTT ATG CCC AGC CCT GGC TC
Immunogen	KLH-conjugated,synthetic peptide corresponding to the C-terminus of human Histone H3.
Classification	Polyclonal
Primary or Secondary	Primary
Test Specificity	Recognizes C-Terminal region of Histone H3, Mr 17kDa

MilliporeSigma™ Upstate™ Histone H2B, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Rabbit
Applications	ChIP Assay,Western Blot
Form	Purified
Gene Accession No.	Q8N257
Includes	The ChIPAb+ Histone H2B set includes the Histone H2B antibody, a negative control rabbit IgG and qPCR primers which amplify a 110bp region of human β-globin promoter.
Antigen	Histone H2B
Regulatory Status	RUO
Gene Symbols	GL105, H2B, H2B-GL105, H2B.1, H2B.q, H2B/q, H2BFQ, MGC119802, MGC119804, MGC129733, MGC129734, OTTHUMP00000013920
Purification Method	Protein A purified
Gene ID (Entrez)	NP_778225
Formulation	Anti-Histone H2B (rabbit polyclonal). One vial containing 25μL of protein A purified rabbit polyclonal in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide, and 30% glycerol. Normal Rabbit IgG. One vial containing 125μg of purified rabbit IgG in 125μL storage buffer. ChIP Primers, β-globin. One vial containing 75μL of 5μM of each primer specific for the human β-globin promoter. FOR: AGG ACA GGT ACG GCT GTC ATC; REV: TTT ATG CCC AGC CCT GGC TC
Immunogen	KLH-conjugated,synthetic peptide corresponding to amino acids 118-126 (CG-AVTKYTSSK) of human Histone H2B,with an N-terminal CG added for conjugation purposes.
Classification	Polyclonal
Primary or Secondary	Primary
Test Specificity	Recognizes and is specific for Histone H2B, MW ∽15kDa. An additional band at ∽23kDa is detected in some samples, which likely corresponds to ubiquityl-Histone H2B.

MilliporeSigma™ Upstate™ NFκB p65 (RelA)α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Mouse
Applications	ChIP Assay,Western Blot
Form	Purified
Gene Accession No.	Q04206
Isotype	IgG3
Includes	This ChIPAb+ NFκB p65 (RelA) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	NFκB p65 (RelA)α
Regulatory Status	RUO
Gene Symbols	RELA, NFKB, p65, p50/p65
Purification Method	Protein A purified
Gene ID (Entrez)	NP_068810.3
Formulation	Anti-NFκB p65 (RelA) (mouse monoclonal). One vial containing 100μg of protein A purified monoclonal IgG3 in a total volume of 143μL in a buffer containing 0.02M phosphate buffer, 0.25M NaCl, pH 7.6 with 0.09% sodium azide, before the addition of glycerol to 30%. Nornal Mouse IgG. One vial containing 125μg of purified mouse IgG in 125μL of storage buffer containing 0.1% sodium azide. ChIP Primers, IĸBα promoter. One vial containing 75μL of 5μM of each primer specific for human IĸBα promoter. FOR: GAC GAC CCC AAT TCA AAT CG; REV: TCA GGC TCG GGG AAT TTC C
Immunogen	Peptide corresponding to human p65 coupled to BSA.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	This antibody recognizes an epitope overlapping the nuclear location signal (NLS) of the p65 subunit of the NFkB heterodimer. Thus it selectively binds to the activated form of NFkB.

MilliporeSigma™ Aquaporin 1 (243-261), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Pricing & Availability
Specifications

Includes	50μL antibody lyophilized from PBS, pH 7.4 and 40μg blocking peptide lyophilized from PBS.
Antigen	Aquaporin 1 (243-261)
Regulatory Status	RUO
Content And Storage	−20°C
Host Species	Rabbit
Applications	Immunoblot,Immunoblot,Immunohistochemistry (Frozen)
Form	Purified
Formulation	50μl antibody lyophilized from PBS, pH 7.4 and 40μg blocking peptide lyophilized from PBS.
Immunogen	a synthetic peptide (KVWTSGQVEEYDLDADDIN) corresponding to amino acids 243-261 of human AQP1,conjugated to KLH
Classification	Polyclonal
Isotype	IgG
Primary or Secondary	Primary
Test Specificity	Recognizes aquaporin-1 in red blood cells. Supplied with AQP1_243-261 blocking peptide (immunogen).

MilliporeSigma™ Upstate ChIPAb+ Sp1 - ChIP Validated Antibody and Primer Set

Full length human Sp1 protein

Pricing & Availability

MilliporeSigma™ Upstate™ Estrogen Receptor αα, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C for one year from date of shipment
Host Species	Mouse
Applications	ChIP Assay,Western Blot
Form	Ascites
Gene Accession No.	P03372
Includes	This ChIPAb+ Estrogen Receptor α -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	Estrogen Receptor αα
Regulatory Status	RUO
Gene Symbols	ESR1, Era, ER-alpha, ESR, ER, NR3A1, ESRA, DKFZp686N23123
Gene ID (Entrez)	NM_000125.2
Formulation	Anti-ERα (mouse ascites). 1 vial containing 100mL ascites. The ERα antibody is made against aa120-170 of bovine estrogen a. It can recognize human and mouse ERα. ChIP primers TFF1 (pS2). 1 vial containing 75mL of 5μM of each control primer specific for human TFF1 (pS2) promoter. FOR: CCG GCC ATC TCT CAC TAT GAA; REV: CCT TCC CGC CAG GGT AAA TAC
Immunogen	The ERα antibody is made against aa 120-170 of bovine estrogen α.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Estrogen Receptor α

MilliporeSigma™ Upstate™ Acetyl-Histone H3, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C from date of receipt
Host Species	Rabbit
Applications	ChIP Assay,Western Blot
Form	Purified
Gene Accession No.	P68431
Includes	Acetyl-Histone H3 ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of H3Ac.
Antigen	Acetyl-Histone H3
Regulatory Status	RUO
Purification Method	Protein A purified
Gene ID (Entrez)	NP_003484
Formulation	Anti-Acetyl-Histone H3 (rabbit polyclonal IgG). One vial containing 125μg of protein A purified antibody in 125μL of storage buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide. Normal Rabbit IgG. One vial containing 125μg of normal rabbit IgG in 125μL volume. Control Primers. One vial containing 75μL of 5μM of each primer specific for for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Immunogen	The acetyl-histone H3 antibody is made against a peptide corresponding to amino acids 1-20 of Tetrahymena histone H3 ARTKQTAR[K]STGG[K]APRKQL(C) where [K] is acetylated)
Classification	Polyclonal
Primary or Secondary	Primary
Test Specificity	Recognizes acetyl-histone H3

MilliporeSigma™ Upstate™ Acetyl-Histone H4, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C from date of receipt
Host Species	Rabbit
Applications	Multiplex,Functional Assay,Immunoassay,Western Blot
Form	Serum
Gene Accession No.	P62805
Includes	This ChIPAb+ Acetyl-Histone H4 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	Acetyl-Histone H4
Regulatory Status	RUO
Gene ID (Entrez)	NP_778224
Formulation	Anti-Acetyl-Histone H4 (rabbit polyclonal IgG). One vial containing 100μL of antiserum containing 0.05% sodium azide. Normal Rabbit Serum. One vial containing 100μL antiserum containing 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM of each primer specific for for human GAPDH.
Immunogen	The acetyl-histone H4 antiserum is made against a peptide corresponding to amino acids 2-19 of Tetrahymena histone H4.
Classification	Polyclonal
Primary or Secondary	Primary
Test Specificity	Recognizes acetylated histone H4 of approximately 10kDa. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly crossreacts with acetylated histone H2B from HeLa cells. May cross-react with other acetylated proteins.

MilliporeSigma™ Upstate™ Phospho-Histone H3 (Ser10)α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Mouse
Applications	ChIP Assay,Western Blot
Form	Purified
Gene Accession No.	P84243
Isotype	IgG
Includes	This ChIPAb+ Phospho-Histone H3 (Ser10) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	Phospho-Histone H3 (Ser10)α
Regulatory Status	RUO
Gene Symbols	H3F3; H3.3B; MGC87782; MGC87783; H3.3A
Purification Method	Protein G purified
Gene ID (Entrez)	NM_003493
Formulation	Anti-phospho-Histone H3 (Ser10) (mouse monoclonal IgG, Clone CMA312). One vial containing 50μg protein G-purified antibody in 50μL PBS containing 0.05% sodium. Normal Mouse IgG. Two vials containing 25μg purified mouse IgG in 25μL storage buffer containing 0.1% sodium azide. Control Primers. One vial containing 75μL of 5μM of each primer specific for the promoter region of human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Immunogen	Immunogen was a synthetic peptide corresponding to amino acids 1-19 of histone H3,phosphorylated at Ser10.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Recognizes histone H3, Mr 17kDa, phosphorylated at Ser10.

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