MilliporeSigma™ Upstate™ Acetyl-Histone H3 (Lys27)α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ 17683

Catalog No. 17-683-MI

Description

Specifically detects Acetyl-Histone H3 (Lys27)α Clone: in Vertebrate samples, and it is validated for Chromatin Immunoprecipitation, Western Blotting

Lysine acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone acetylation is often associated with transcriptional activation and acetylation of H3K27 is found at active enhancers.

Specifications

• Antigen: Acetyl-Histone H3 (Lys27)α
• Classification: Monoclonal
• Formulation: Anti-acetyl-Histone H3 (Lys27) (mouse monoclonal IgG1, Clone CMA309). One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium. Normal Mouse IgG. Two vials containing 25μg purified Mouse IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers, RPL10 Promoter. One vial containing 75μL of 5μM of each primer specific for the promoter region of human RPL10. FOR: ACC CGT CTT CGA CAG GAC T; REV: GGA ACG GAA GAC GAG AAC AG
• Host Species: Mouse
• Quantity: 25 Assays
• Research Discipline: Epigenetics and Nuclear Function
• Gene ID (Entrez): NM_003493
• Includes: Acetyl-Histone H3 (Lys27) ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of H3K27Ac.
• Content And Storage: −20°C for up to 12 months; Avoid repeated freeze/thaw cycles
• Isotype: IgG

• Applications: ChIP Assay, Western Blot
• Conjugate: Unconjugated
• Gene Accession No.: Q66I33
• Immunogen: The acetyl-histone H3 (Lys27) purified antibody is made against a synthetic peptide (acetylated at Lys27) corresponding to amino acids 19-37 of histone H3.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes histone H3, Mr 17kDa, acetylated at Lys27.
• Target Species: Vertebrates
• Form: Purified

For Research Use Only