MilliporeSigma™ Upstate™ Dimethyl-Histone H3 (Lys9), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ 17648

Catalog No. 17-648-MI

Description

Specifically detects Dimethyl-Histone H3 (Lys9) Clone: in Human, Mammalia, Mouse samples, and it is validated for Chromatin Immunoprecipitation, Immunoprecipitation, Western Blotting

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression.

Specifications

• Antigen: Dimethyl-Histone H3 (Lys9)
• Classification: Polyclonal
• Formulation: Dimethyl-Histone H3 (Lys9) (rabbit polyclonal serum). One vial containing 100μL of antiserum containing 0.05% sodium azide.
  Normal Rabbit Serum. One vial containing 100μL antiserum containing 0.05% sodium azide.
  ChIP Primers, β-Globin. One vial containing 75μL of 5μM of each primer specific for human β-globin. FOR: AGG ACA GGT ACG GCT GTC ATC REV: TTT ATG CCC AGC CCT GGC TC
• Gene Symbols: H3F3A, H3F3B, H3F3
• Immunogen: The dimethyl-histone H3 (Lys9) rabbit serum is made against a KLH-conjugated, branched synthetic peptide containing the sequence ..Rme2KSTG.., in which me2K corresponds to dimethyl-lysine at residue 9 of human histone H3
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Dimethyl-Histone H3 (Lys9)
• Target Species: Human, Mammalia, Mouse
• Form: Serum

• Applications: ChIP Assay, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Gene Accession No.: Q66I33
• Host Species: Rabbit
• Quantity: 25 Assays
• Research Discipline: Epigenetics and Nuclear Function
• Gene ID (Entrez): NM_003493
• Includes: Dimethyl-Histone H3 (Lys9) ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of H3K9Me2.
• Content And Storage: −20°C from date of receipt

For Research Use Only