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MilliporeSigma™ Upstate™ Histone H2B, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ 1710054

Catalog No. 17-100-54MI

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Specifically detects Histone H2B Clone: in Chicken, Human, S. cerevisiae, Xenopus, Yeast samples, and it is validated for Chromatin Immunoprecipitation, Western Blotting

Histone H2B is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the 'beads on a string' structure.


Histone H2B
Anti-Histone H2B (rabbit polyclonal). One vial containing 25μL of protein A purified rabbit polyclonal in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide, and 30% glycerol. Normal Rabbit IgG. One vial containing 125μg of purified rabbit IgG in 125μL storage buffer. ChIP Primers, β-globin. One vial containing 75μL of 5μM of each primer specific for the human β-globin promoter. FOR: AGG ACA GGT ACG GCT GTC ATC; REV: TTT ATG CCC AGC CCT GGC TC
GL105, H2B, H2B-GL105, H2B.1, H2B.q, H2B/q, H2BFQ, MGC119802, MGC119804, MGC129733, MGC129734, OTTHUMP00000013920
KLH-conjugated, synthetic peptide corresponding to amino acids 118-126 (CG-AVTKYTSSK) of human Histone H2B, with an N-terminal CG added for conjugation purposes.
25 Assays
Epigenetics and Nuclear Function
The ChIPAb+ Histone H2B set includes the Histone H2B antibody, a negative control rabbit IgG and qPCR primers which amplify a 110bp region of human β-globin promoter.
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
ChIP Assay, Western Blot
Protein A purified
Recognizes and is specific for Histone H2B, MW ∽15kDa. An additional band at ∽23kDa is detected in some samples, which likely corresponds to ubiquityl-Histone H2B.
Chicken, Human, S. cerevisiae, Xenopus species, Yeast


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