Protein Purification Reagents and Kits
Protein Purification Reagents and Kits
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Filtered Search Results
Cytiva HisTrap™ FF Columns: 5 x 5mL
Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.
Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Cytiva PD MidiTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL. Cytiva Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Cytiva PD MiniTrap™ G-10 Sample Preparation Columns
Use for buffer exchange and cleanup of biological samples, for example peptides, small proteins, and oligosaccharides. Cytiva Life Sciences™ PD MiniTrap™ G-10 Sample Preparation Columns are prepacked, single-use gravity columns.
Cytiva XK Chromatography Columns (Empty)
Ensure trouble-free operation for low to medium pressure. Cytiva XK Chromatography Columns (Empty) connect directly to ÄKTA design instruments or other liquid chromatography systems. The user-friendly ensures reproducible, accurate packing.
Particle Size | 10μm pore size |
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Product Type | XK Chromatography Columns (Empty) |
Column Type | Empty Chromatography Column |
Material | Borosilicate glass |
For Use With (Application) | Liquid chromatography at low to medium pressure |
Support Type | Polypropylene (reinforced glass fiber)/Polyamide Net |
Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
Flow Rate | <20 mL/min. |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Diameter (English) Inner | 0.6 in. |
Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Diameter (Metric) Inner | 16 mm |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Cytiva Glutathione Sepharose™ 4B Media
Use for high capacity purification of Glutathione S-Transferase (GST) tagged fusion proteins. Cytiva Life Sciences™ Glutathione Sepharose™ 4B Media requires only a single-step for purification.
Particle Size | 90 μm |
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pH Range | 4 to 13 |
Material | Glutathione Sepharose 4 B |
Cytiva LabMate™ PD-10 Buffer Reservoirs
Use for high-recovery desalting, buffer exchange and removal of low-molecular weight compounds packed with Sephadex™ G-25 Medium. Cytiva Life Sciences™ LabMate™ PD-10 Buffer Reservoirs provide fast, affordable performance.
Cytiva HisTrap™ excel Prepacked Columns
For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.
Type | Pre-packed Column |
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Content And Storage | 4°C to 30°C |
Description | HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 2 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Cytiva Ni Sepharose™ 6 Fast Flow
Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.
Particle Size | 90μm |
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Buffer | 0.1M HCl, 0.1M NaOH, 8M Urea |
Flow Rate | 600cm/hr. |
Height (Metric) | 250mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | Ni Sepharose 6 FF chromatography media |
dimMatrix | Ni-Sepharose |
Support Type | 6% Cross-linked Agarose |
Max. Pressure | 1 bar |
Height (English) | 9.84 in. |
Cytiva Streptavidin Sepharose High Performance Resin
Affinity chromatography resin designed for fast and reliable, high resolution purification of biotinylated biomolecules.
Cytiva Tagged Column Package, His (2-step Purification Protocol)
Combines affinity with size exclusion chromatography to obtain highly pure his-tagged proteins
Cytiva Gelatin Sepharose™ 4B Media
Designed for the purification or depletion of fibronectin as gelatin binds to fibronectin with high specificit
Column Type | Column Accesory |
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Quantity | 2 Pack |
Material | Polypropylene |