Test
Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.
Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium.
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL. Cytiva Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Use for buffer exchange and cleanup of biological samples, for example peptides, small proteins, and oligosaccharides. Cytiva Life Sciences™ PD MiniTrap™ G-10 Sample Preparation Columns are prepacked, single-use gravity columns.
For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.
Type | Pre-packed Column |
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Content And Storage | 4°C to 30°C |
Description | HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 2 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.
Particle Size | 90μm |
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Buffer | 0.1M HCl, 0.1M NaOH, 8M Urea |
Flow Rate | 600cm/hr. |
Height (Metric) | 250mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | Ni Sepharose 6 FF chromatography media |
dimMatrix | Ni-Sepharose |
Support Type | 6% Cross-linked Agarose |
Max. Pressure | 1 bar |
Height (English) | 9.84 in. |
Ensure trouble-free operation for low to medium pressure. Cytiva XK Chromatography Columns (Empty) connect directly to ÄKTA design instruments or other liquid chromatography systems. The user-friendly ensures reproducible, accurate packing.
Particle Size | 10μm pore size |
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Product Type | XK Chromatography Columns (Empty) |
Column Type | Empty Chromatography Column |
Material | Borosilicate glass |
For Use With (Application) | Liquid chromatography at low to medium pressure |
Support Type | Polypropylene (reinforced glass fiber)/Polyamide Net |
Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.
Particle Size | 34 μm |
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Ligand Type | 10mg Heparin/mL medium |
Height (Metric) | 2.5cm |
Content And Storage | 4° to 30°C |
pH Range | 5 to 10 |
Product Type | Heparin HP Column |
For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
Sample Type | DNA-binding Proteins, coagulation facto rs, Lipoproteins and Protein synthesis facto rs |
Material | Polypropylene |
For Use With (Application) | Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins |
Support Type | 6% Highly Cross-linked Agarose |
Max. Pressure | 42psi |
Height (English) | 0.98 in. |
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
Flow Rate | <20 mL/min. |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Diameter (English) Inner | 0.6 in. |
Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Diameter (Metric) Inner | 16 mm |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Use for high capacity purification of Glutathione S-Transferase (GST) tagged fusion proteins. Cytiva Life Sciences™ Glutathione Sepharose™ 4B Media requires only a single-step for purification.
Particle Size | 90 μm |
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pH Range | 4 to 13 |
Material | Glutathione Sepharose 4 B |
Use for high-recovery desalting, buffer exchange and removal of low-molecular weight compounds packed with Sephadex™ G-25 Medium. Cytiva Life Sciences™ LabMate™ PD-10 Buffer Reservoirs provide fast, affordable performance.
Affinity chromatography resin designed for fast and reliable, high resolution purification of biotinylated biomolecules.
Designed for intermediate purification and polishing of viruses and other large biomolecules