Test
Horse Polyclonal Secondary Antibody
Content And Storage | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
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Target Species | Mouse |
Host Species | Equine |
Conjugate | Biotin |
Applications | Immunocytochemistry,Immunohistochemistry,In Situ Hybridization (ISH),Western Blot |
Form | Liquid |
Isotype | IgG |
Concentration | 1.5 mg/mL |
Antigen | Mouse IgG (H+L) |
Regulatory Status | RUO |
Purification Method | Affinity chromatography |
Formulation | PBS with 3mg/mL BSA and 0.08% sodium azide; pH 7.8 |
Classification | Polyclonal |
Primary or Secondary | Secondary |
Rabbit Polyclonal Antibody has been used in 1 publication
Content And Storage | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
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Target Species | Mouse |
Host Species | Equine |
Conjugate | Unconjugated |
Applications | ELISA,Immunocytochemistry,Immunohistochemistry,In Situ Hybridization (ISH),Western Blot |
Form | Liquid |
Isotype | IgG |
Concentration | 3 mg/mL |
Antigen | Mouse IgG (H+L) |
Regulatory Status | RUO |
Purification Method | Affinity chromatography |
Formulation | PBS with 20mg/mL sucrose and 0.08% sodium azide; pH 7.8 |
Classification | Polyclonal |
Primary or Secondary | Secondary |
Anti-mouse IgG, HRP-linked Antibody 100 µl
Biotin-SP-AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Sw Sr Prot)
The ImmPRESS-HRP Anti-Mouse IgG (Horseradish Peroxidase) Polymer Detection Kit is made in horse and targets mouse primary antibodies. This IHC staining system uses novel conjugation chemistry to create a highly sensitive, ready-to-use, one-step, biotin-free detection system. (A combination of highly active enzyme (peroxidase or alkaline phosphatase) and affinity-purified secondary antibodies results in crisp, strong staining. The ImmPRESS micropolymer technology minimizes steric interference and provides enhanced accessibility to the primary antibody targets. The IHC immunostaining protocol is simple. Following a blocking step with 2.5% horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with a peroxidase or alkaline phosphatase substrate. The ImmPRESS Reagent is ready-to-use and stains 250-500 sections.
The ImmPRESS-AP Anti-Rabbit IgG (Alkaline Phosphatase) Polymer Detection Kit is made in horse and targets rabbit primary antibodies. This staining system uses novel conjugation chemistry to create a highly sensitive, ready-to-use, one-step, biotin-free detection system. (A combination of highly active enzyme (peroxidase or alkaline phosphatase) and affinity-purified secondary antibodies results in crisp, strong staining. The ImmPRESS micropolymer technology minimizes steric interference and provides enhanced accessibility to the primary antibody targets. The IHC immunostaining protocol is simple. Following a blocking step with 2.5% horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with a peroxidase or alkaline phosphatase substrate. The ImmPRESS Reagent is ready-to-use and stains 75-150 sections.
Vector Laboratories secondary antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots. Biotinylated Horse Anti-Mouse IgG (H+L) is supplied in solution and recognizes both heavy and light chains. With some exceptions, the recommended dilution for most applications is 1:200.
Anti-mouse IgG, HRP-linked Antibody 1 ml
Horse Anti-Goat IgG Antibody (H+L), DyLight 488, 1.5 mg, is an affinity-purified, ultrapure, high-affinity antibody that has been thoroughly adsorbed against serum and immunoglobulins from potentially interfering species. Unless otherwise specified, antibodies recognize both heavy and light chains (H+L). Optimally labeled with DyLight 488 to provide the brightest label for fluorescence microscopy. Supplied in solution. Excitation: 493 nm; Emission: 518 nm; Color: Green.
Horse Anti-Rabbit IgG Antibody (H+L), DyLight 488, 1.5 mg, is an affinity-purified, ultrapure, high-affinity antibody. It is thoroughly adsorbed against serum and immunoglobulins from potentially interfering species. Unless otherwise specified, antibodies recognize both heavy and light chains (H+L). Optimally labeled with DyLight 488 to provide the brightest label for fluorescence microscopy. Supplied in solution. Excitation: 493 nm; Emission: 518 nm; Color: Green.
NC2605271 ANTI MOUSE DYLIGHT488
NC2828575 DEXTRAN CY5
Vector Laboratories secondary antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. This HRP Horse Anti-Mouse IgG Antibody is cross-linked with high specific activity horseradish peroxidase. The antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots.
50-234-0497 READIUSE CL-APC CROSS LINKED