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Horse anti-Mouse IgG (H+L) Secondary Antibody, Invitrogen™

Horse Polyclonal Secondary Antibody

Supplier:  Thermo Scientific 31181

Catalog No. PI31181


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Description

Description

Product # 31181 has been successfully used in Western blot, IF, ICC, IHC, ELISA and ISH applications. Product # 31181 reacts with heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins. Based on solid-phase enzyme immunoassay, this antibody cross-reacts with rat IgG and mouse IgM (25%), hamster IgG (12%), guinea pig IgG and rat IgM (6%), bovine IgM, dog IgM, goat IgM, human IgM, Rhesus monkey IgG, sheep IgG, sheep IgM, swine IgG (1%). It has minimum cross-reactivity with immunoglobulins from other species. The activity measured by a solid phase binding assay is 94%.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Mouse IgG (H+L)
Polyclonal
Unconjugated
Equine
1.5 mg
Secondary
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
IgG
ELISA, Immunocytochemistry, Immunohistochemistry, In Situ Hybridization (ISH), Western Blot
3 mg/mL
PBS with 20mg/mL sucrose and 0.08% sodium azide; pH 7.8
Affinity chromatography
RUO
Mouse
Liquid
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