Test
Content And Storage | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
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Target Species | Mouse |
Host Species | Equine |
Conjugate | Unconjugated |
Applications | ELISA,Immunocytochemistry,Immunohistochemistry,In Situ Hybridization (ISH),Western Blot |
Form | Liquid |
Isotype | IgG |
Concentration | 3 mg/mL |
Antigen | Mouse IgG (H+L) |
Regulatory Status | RUO |
Purification Method | Affinity chromatography |
Formulation | PBS with 20mg/mL sucrose and 0.08% sodium azide; pH 7.8 |
Classification | Polyclonal |
Primary or Secondary | Secondary |
Rabbit Polyclonal Antibody has been used in 1 publication
Horse Polyclonal Secondary Antibody
Content And Storage | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
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Target Species | Mouse |
Host Species | Equine |
Conjugate | Biotin |
Applications | Immunocytochemistry,Immunohistochemistry,In Situ Hybridization (ISH),Western Blot |
Form | Liquid |
Isotype | IgG |
Concentration | 1.5 mg/mL |
Antigen | Mouse IgG (H+L) |
Regulatory Status | RUO |
Purification Method | Affinity chromatography |
Formulation | PBS with 3mg/mL BSA and 0.08% sodium azide; pH 7.8 |
Classification | Polyclonal |
Primary or Secondary | Secondary |
Anti-mouse IgG, HRP-linked Antibody 100 µl
Vector Laboratories secondary antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots. Biotinylated Horse Anti-Mouse IgG (H+L) is supplied in solution and recognizes both heavy and light chains. With some exceptions, the recommended dilution for most applications is 1:200.
The ImmPRESS-HRP Anti-Mouse IgG (Horseradish Peroxidase) Polymer Detection Kit is made in horse and targets mouse primary antibodies. This IHC staining system uses novel conjugation chemistry to create a highly sensitive, ready-to-use, one-step, biotin-free detection system. (A combination of highly active enzyme (peroxidase or alkaline phosphatase) and affinity-purified secondary antibodies results in crisp, strong staining. The ImmPRESS micropolymer technology minimizes steric interference and provides enhanced accessibility to the primary antibody targets. The IHC immunostaining protocol is simple. Following a blocking step with 2.5% horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with a peroxidase or alkaline phosphatase substrate. The ImmPRESS Reagent is ready-to-use and stains 250-500 sections.
ImmPRESS-AP Goat Anti-Rat IgG Polymer Detection Kit, Alkaline Phosphatase
50-234-0527 READIUSE CL-APC CROSS LINKED
Goat Anti-Human Lambda Chain Antibody, Fluorescein, 0.5 mg, is prepared by hyperimmunizing animals in a manner that produces high-affinity antibodies. These are then purified by affinity chromatography to remove any low-affinity antibodies and by solid-phase adsorption techniques to remove cross-reactivities. The final product is subjected to rigorous QC, including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis, and solid-phase binding assays. Conjugation ensures the maximum degree of labeling without compromising antibody specificity or affinity. The labeled antibody then undergoes further QC, including immunohistochemical analysis. This chain-specific antibody is produced specifically to distinguish between chains or classes of target immunoglobulins and has virtually no cross-reactivity with other immunoglobulin classes or other heavy or light chains. Fluorescein has an excitation maximum of 495 nm and an emission maximum of 515 nm.
NC2828567 DEXTRAN CY5
Fluorescein (FITC)-AffiniPure Goat Anti-Mouse IgG, Fc Subclass 1 Specific (min X Hu,Bov,Rb Sr Prot)
Goat Anti-Rabbit IgG Antibody (H+L), AMCA, 1.5 mg, is prepared by hyperimmunizing animals in a manner that produces high-affinity antibodies. These are then purified by affinity chromatography to remove any low-affinity antibodies and by solid-phase adsorption techniques to remove cross-reactivities. The final product is subjected to rigorous QC, including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis, and solid-phase binding assays. During antibody labeling, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes further QC, including immunohistochemical analysis. This antibody has been optimally labeled with AMCA to provide the brightest label for fluorescence microscopy. AMCA has an excitation maximum of 350 nm and an emission maximum of 450 nm.
The ImmPRESS-AP Anti-Rabbit IgG (Alkaline Phosphatase) Polymer Detection Kit is made in horse and targets rabbit primary antibodies. This staining system uses novel conjugation chemistry to create a highly sensitive, ready-to-use, one-step, biotin-free detection system. (A combination of highly active enzyme (peroxidase or alkaline phosphatase) and affinity-purified secondary antibodies results in crisp, strong staining. The ImmPRESS micropolymer technology minimizes steric interference and provides enhanced accessibility to the primary antibody targets. The IHC immunostaining protocol is simple. Following a blocking step with 2.5% horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with a peroxidase or alkaline phosphatase substrate. The ImmPRESS Reagent is ready-to-use and stains 75-150 sections.
Anti-mouse IgG, HRP-linked Antibody 5 ml
Biotin-SP-conjugated AffiniPure Alpaca Anti-Rabbit IgG (H+L) (min X Bov,Hu,Ms Sr Prot).