Thermo Scientific™ Phusion Blood Direct PCR Kit

Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

Supplier:  Thermo Scientific™ F547S

Catalog No. F547S

Description

Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase exhibits high resistance to PCR inhibitors found in blood and retains polymerase activity even with samples containing 40% whole blood.

This DNA polymerase exhibits exceptional accuracy in proofreading and makes the Phusion Blood Direct PCR Kit one of the best choices for demanding applications, such as cloning. Phusion Blood Direct PCR Kit is versatile and also permits analysis of blood samples from various species and with different anticoagulants or storage conditions.

Highlights

• No need for time-consuming and expensive DNA purification steps
• Simple protocol requires minimal hands-on time
• Extremely short PCR protocol times
• Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product

The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases.

For optimal results start by accurately calculating your Tm with our Tm calculator.

Specifications

• Content And Storage: • Phusion Blood II DNA Polymerase, 40 μL
  • 2X Phusion Blood PCR Buffer with dNTPs and MgCl₂, 1.25 mL
  • Universal Control Primer Mix, 40 μL
  • 50 mM EDTA, 0.5 mL
  • 50 mM MgCl₂, 1.5 mL
  • DMSO, 0.5 mL
  
  Store at -20°C.
• PCR Method: Direct PCR
• Reaction Speed: Fast
• Shipping Condition: Wet or Dry Ice
• Hot Start: Built-In Hot Start
• Overhang: Blunt
• Sample Type: Blood

• GC-Rich PCR Performance: High
• Polymerase: Phusion Blood II DNA Polymerase
• Product Type: Direct PCR Kit
• Concentration: 2 U/μL
• No. of Reactions: 100 Reactions
• Reaction Format: Separate Components
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.