Thermo Scientific™ Phusion Blood Direct PCR Kit

Perfrom PCR directly from whole blood without DNA purification.

Supplier:  Thermo Scientific™ F547S

Catalog No. F547S

Description

Phusion Blood Direct PCR Kit is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to PCR inhibitors present in blood and retains its activity even with samples containing 40% whole blood.

This extremely accurate proofreading DNA polymerase makes the Phusion Blood Direct PCR Kit an ideal choice even for demanding applications, such as cloning. Phusion Blood Direct PCR Kit also allows analysis of blood samples with different anticoagulants, storage conditions and from various species.

Phusion Blood Direct PCR Kit is designed for amplification of DNA from whole blood without a prior purification step. The kit utilizes a modified Phusion Hot Start II High-Fidelity DNA Polymerase. This highly processive and robust PCR enzyme is resistant to the inhibitors present in blood and retains its activity even at 40% blood concentration. Phusion Blood Direct PCR Kit allows analysis of blood samples with different anticoagulants, storage conditions and from various species.

• No need for DNA extraction
• Allows up to 40% blood in the PCR reaction
• Tolerates heparin, citrate, and EDTA, and is compatible with frozen blood or blood stored on various types of filter papers
• Modified Phusion Hot Start II DNA Polymerase gives specific and accurate results with high yields and short protocol times

Includes:

Phusion Blood DNA Polymerase, 2x Phusion Blood PCR Buffer (includes dNTPs and MgCl₂), EDTA, MgCl₂, DMSO, universal control primer mix

Recommended for:

Detection of SNPs with PCR-RFLP. Genotyping.

Specifications

• Content And Storage: Contains:
  • 40 μL Phusion Blood II DNA Polymerase
  • 1.25 mL 2X Phusion Blood PCR Buffer (includes dNTPs and MgCl₂)
  • 40 μL Universal Control Primer Mix
  • 0.5 mL 50 mM EDTA
  • 1.5 mL 50 mM MgCl₂
  • 0.5 mL DMSO
  
  Store at -20°C.
• PCR Method: Direct PCR
• Reaction Speed: Fast
• Product Type: Direct PCR Kit
• Hot Start: Built-In Hot Start
• Overhang: Blunt
• Sample Type: Blood

• GC-Rich PCR Performance: High
• Polymerase: Phusion Blood II DNA Polymerase
• Product Line: Phusion
• Quantity: 100 x 20 μL Reactions
• No. of Reactions: 100 Reactions
• Reaction Format: Separate Components
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.